Jolivet M E, Audibert F M, Gras-Masse H, Tartar A L, Schlesinger D H, Wirtz R, Chedid L A
Infect Immun. 1987 Jun;55(6):1498-502. doi: 10.1128/iai.55.6.1498-1502.1987.
Four synthetic peptides that copy fragments of two bacterial antigens (Streptococcus pyogenes M protein and diphtheria toxin), one viral antigen (hepatitis B surface antigen), and one parasitic antigen (circumsporozoite protein of Plasmodium knowlesi) were covalently bound within the same construct. This totally synthetic polyvalent administered to mice with Freund complete adjuvant or in saline with murabutide (an adjuvant-active muramyl peptide) elicited high levels of antibodies which, in certain cases, were shown to be biologically active. The results indicated that these antibodies recognized specifically the four peptides. None of the epitopes were immunodominant. It was also demonstrated that the association of several peptides enhanced their respective immunogenicities as compared with those of their homopolymers. Finally, this study shows that a totally synthetic vaccine administered in saline with a synthetic adjuvant can be immunogenic in the absence of a protein carrier.
四种合成肽分别复制了两种细菌抗原（化脓性链球菌M蛋白和白喉毒素）、一种病毒抗原（乙肝表面抗原）以及一种寄生虫抗原（诺氏疟原虫环子孢子蛋白）的片段，并在同一构建体中进行共价结合。这种完全合成的多价肽与弗氏完全佐剂一起或与murabutide（一种具有佐剂活性的胞壁酰肽）在生理盐水中一同给予小鼠后，引发了高水平的抗体，在某些情况下，这些抗体显示出生物活性。结果表明，这些抗体能够特异性识别这四种肽。没有一个表位具有免疫优势。研究还表明，与各自的同聚物相比，几种肽的组合增强了它们各自的免疫原性。最后，这项研究表明，在生理盐水中与合成佐剂一起给予的完全合成疫苗在没有蛋白质载体的情况下也具有免疫原性。
