Raiders Stephan, Klein Mason, Singhvi Aakanksha
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, USA.
Molecular and Cellular Biology Graduate Program, University of Washington, USA.
Bio Protoc. 2022 Apr 5;12(7):e4370. doi: 10.21769/BioProtoc.4370.
Thermotaxis behaviors in exhibit experience-dependent plasticity of thermal preference memory. This behavior can be assayed either at population level, on linear temperature gradients, or at the individual animal level, by radial isothermal or microfluidic tracking of orientation. These behaviors are low-throughput as well as variable, due to the inherent sensitivity to environmental perturbations. To facilitate reproducible studies, we describe an updated apparatus design that enables simultaneous runs of three thermal preference assays, instead of single-run assays described previously. By enabling parallel runs of control and experimental conditions, this set-up enables more throughput and rigorous assessment of behavioral variability.
在[具体生物]中，趋温行为表现出热偏好记忆的经验依赖性可塑性。这种行为可以在群体水平上，在线性温度梯度上进行测定，也可以在个体动物水平上，通过径向等温或微流体定向追踪来测定。由于对环境扰动具有内在敏感性，这些行为通量较低且存在变异性。为了促进可重复的研究，我们描述了一种更新的仪器设计，该设计能够同时进行三次热偏好测定，而不是先前描述的单次测定。通过实现对照和实验条件的并行运行，这种设置能够实现更高的通量，并对行为变异性进行更严格的评估。
