Mostafazadeh Mostafa, Sadeghi Heibatollah, Sadeghi Hossein, Zarezade Vahid, Hadinia Abolghasem, Panahi Kokhdan Esmaeel
Department of Biochemistry and Clinical Laboratories, Tabriz University of Medical Sciences, Tabriz, I.R. Iran.
Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, I.R. Iran.
Res Pharm Sci. 2022 Apr 18;17(3):305-314. doi: 10.4103/1735-5362.343084. eCollection 2022 Jun.
Previously, we reported the anti-inflammatory properties of (watercress) in several models of acute inflammation. This study was designed to explore the effects of topical and systemic administrations of in the two chronic inflammatory models and to evaluate the role of TNF-α and IL-1β in these effects.
Folin-Ciocalteu and aluminum chloride methods were used to estimate the extract's total phenol and flavonoid content, respectively. Carrageenan-induced paw edema was carried out and TNF-α and IL-1β concentrations in the carrageenan-treated paw tissue were determined. Formalin injection into rat hind paws (7 days) and the application of 12-O-tetradecanoyl phorbol-13-acetate (TPA) on mouse ears (9 days) were used to simulate chronic inflammation. Furthermore, a histological assessment of the inflamed tissues was carried out.
FINDINGS/RESULTS: The extract's flavonoid and phenolic contents were 90.26 ± 4.81 mg rutin equivalents/g and 68 ± 8.16 gallic acid equivalents/g gallic acid, respectively. pretreatment in all doses administered considerably decreased carrageenan-induced edema. The extract also reduced IL-1β levels in carrageenan- treated paws while did not affect TNF-α levels. Oral and topical administrations of considerably reserved the paw and ear edema. The extract also ameliorated the tissue injuries due to formalin and TPA challenges.
The data confirmed the topical and systemic anti-inflammatory effects of watercress against two chronic models of inflammation. They suggested that these properties are not related to TNF-α but could be attributed to inhibition of IL-1β and inhibition of leukocyte infiltration.
此前，我们已在多种急性炎症模型中报道了豆瓣菜的抗炎特性。本研究旨在探究豆瓣菜局部和全身给药在两种慢性炎症模型中的作用，并评估肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）在这些作用中的角色。
分别采用福林-西奥尔特法和氯化铝法来估算提取物中的总酚和黄酮含量。进行角叉菜胶诱导的爪肿胀实验，并测定角叉菜胶处理的爪组织中TNF-α和IL-1β的浓度。向大鼠后爪注射福尔马林（7天）以及在小鼠耳部涂抹12-O-十四酰佛波醇-13-乙酸酯（TPA）（9天），以模拟慢性炎症。此外，还对炎症组织进行了组织学评估。
提取物中的黄酮和酚类含量分别为90.26±4.81毫克芦丁当量/克和68±8.16没食子酸当量/克没食子酸。所有给药剂量的豆瓣菜预处理均显著减轻了角叉菜胶诱导的肿胀。提取物还降低了角叉菜胶处理爪中的IL-1β水平，但未影响TNF-α水平。豆瓣菜的口服和局部给药均显著减轻了爪和耳部的肿胀。提取物还改善了福尔马林和TPA刺激引起的组织损伤。
数据证实了豆瓣菜对两种慢性炎症模型具有局部和全身抗炎作用。研究表明，这些特性与TNF-α无关，可能归因于对IL-1β的抑制以及对白细胞浸润的抑制。
