Han Yue, Zhang Ai-Hua, Zhang Ying-Zhi, Sun Hui, Meng Xiang-Cai, Wang Xi-Jun
Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine Heping Road 24 Harbin 150040 China
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology Avenida Wai Long, Taipa Macau China.
RSC Adv. 2018 Mar 28;8(22):11983-11990. doi: 10.1039/c8ra01029c. eCollection 2018 Mar 26.
Recent advances in the study of high-throughput metabolomics combined with high-resolution mass spectrometry have accelerated our understanding of the efficacy, mechanisms, and application of natural products. In this study, we have used chemical metabolomics to investigate and discover small molecule metabolites for the potential mechanism of Harms leaf (ASL) against acute promyelocytic leukemia (APL). Based on high-throughput metabolomics, the underlying biomarker was found by combining chromatography coupled with quadrupole time-of-flight mass spectrometry with multivariate data analysis. The protective effect of ASL was dissected using biochemical indicators, pathology sections, immunohistochemistry, and multivariate analysis. Furthermore, 13 potential biomarkers associated with the pathway of sugar metabolism, amino-acid metabolism, nucleotide metabolism, and the metabolism of arachidonic acid were identified from serum samples. This study would help to understand chemical metabolomics for investigating the anti-APL effectiveness of ASL.
高通量代谢组学与高分辨率质谱联用研究的最新进展，加速了我们对天然产物的功效、作用机制及应用的理解。在本研究中，我们运用化学代谢组学来研究和发现小分子代谢物，以探讨青蒿叶（ASL）抗急性早幼粒细胞白血病（APL）的潜在机制。基于高通量代谢组学，通过将色谱与四极杆飞行时间质谱联用并结合多变量数据分析，发现了潜在的生物标志物。利用生化指标、病理切片、免疫组织化学和多变量分析剖析了ASL的保护作用。此外，从血清样本中鉴定出了13种与糖代谢、氨基酸代谢、核苷酸代谢及花生四烯酸代谢途径相关的潜在生物标志物。本研究将有助于理解化学代谢组学在研究ASL抗APL有效性方面的作用。
