Department of Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Skłodowska Street, 85-094 Bydgoszcz, Poland.
Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland.
Oxid Med Cell Longev. 2019 Mar 10;2019:8673521. doi: 10.1155/2019/8673521. eCollection 2019.
Secondary metabolites of the roots of spp. cultivated in Poland, or the bioactivity, are not fully known. The 75% methanol extracts of five spp. (, , , , and ) were examined for the content of polyphenols and phenolic acids as well as for antiacetylcholinesterase, antihyaluronidase, anti-DPPH, and cytotoxic activities. The richest in polyphenols were the roots of (10.4 mg/g DW), while in flavonoids the roots of (6.5 mg/g DW). The richest in phenolic acids occurred the roots of [protocatechuic acid (1865 g/g DE), caffeic acid (244 g/g DE), and -coumaric and ferulic acids (55 g/g DE)]. The highest inhibition of AChE was observed for and (32%), at the concentration of 100 g/0.19 mL of the reaction mixture, while that of Hyal for the roots of (40.7%), at the concentration of 100 g/0.16 mL of the reaction mixture. Among five species tested, the extract exhibited the strongest HL-60 cell line growth's inhibition (IC 270 g/mL). The extracts reduced DPPH in a time-dependent mode, at the concentration of 0.8 mg/mL. After 90 min from 14.7 to 26.2%, DPPHwas reduced. A phytochemical composition and activity of the species, cultivated in Poland, are still under research; however, on the basis of the results obtained, it may be concluded that they may become a source of phytochemicals and be useful for Europe's citizens.
波兰种植的 spp. 的次生代谢产物（次级代谢物）及其生物活性尚未完全阐明。本文对 5 种 spp.（ 、 、 、 、 ）的 75%甲醇提取物的多酚和酚酸含量以及抗乙酰胆碱酯酶、抗透明质酸酶、抗 DPPH 和细胞毒性活性进行了研究。其中， 根中的多酚含量最为丰富（10.4mg/g DW），而 根中的类黄酮含量最为丰富（6.5mg/g DW）。根中酚酸含量最为丰富的是 （原儿茶酸 1865g/g DE、咖啡酸 244g/g DE 以及 -香豆酸和阿魏酸 55g/g DE）。在浓度为 100g/0.19mL 反应混合物时， 根和 根对乙酰胆碱酯酶（AChE）的抑制作用最强（32%），在浓度为 100g/0.16mL 反应混合物时， 根对透明质酸酶（Hyal）的抑制作用最强（40.7%）。在测试的 5 个物种中， 提取物对 HL-60 细胞系生长的抑制作用最强（IC 270g/mL）。提取物以时间依赖性方式降低 DPPH，在浓度为 0.8mg/mL 时，90min 后 DPPH 降低了 14.7%至 26.2%。波兰种植的 spp. 的植物化学成分和活性仍在研究中；然而，根据所获得的结果，可以得出结论，它们可能成为植物化学物质的来源，并对欧洲公民有用。
