Liang Fupeng, Qiao Yi, Duan Mengqin, Ju An, Lu Na, Li Junji, Tu Jing, Lu Zuhong
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University No. 2 Si Pai Lou Nanjing 210096 China
Department of Bioengineering, Stanford University 443 Via Ortega Stanford CA 94305 USA.
RSC Adv. 2018 Feb 27;8(16):8732-8738. doi: 10.1039/c7ra13334k. eCollection 2018 Feb 23.
Polypropylene (PP) material has been widely used in the biomedical field for decades due to its high reliability in biochemical reactions. However, a pure PP material microfluidic chip has rarely been reported. Herein, a simple and rapid method has been developed to fabricate a pure PP microfluidic chip by a thermal bonding process using a PP film and PP substrate. An experiment for two-temperature PCR in the pure PP microfluidic system without a pre-treatment process has been successfully carried out. It is shown that the PP microfluidic chip has a high structural strength, tightness for water sealing, and low nonspecific adsorption, which promotes the practical application of microfluidics in the biomedical field. Compared to other existing microfluidic chip technologies, our pure PP material microfluidic chip and its fabrication method have many advantages such as high-speed production rate and extremely low cost. It can be achieved in industrial assembly lines for standardized manufacturing.
几十年来，聚丙烯（PP）材料因其在生化反应中的高可靠性而在生物医学领域得到广泛应用。然而，很少有关于纯PP材料微流控芯片的报道。在此，我们开发了一种简单快速的方法，通过使用PP膜和PP基板的热键合工艺来制造纯PP微流控芯片。在未经预处理的纯PP微流控系统中成功进行了双温度PCR实验。结果表明，PP微流控芯片具有较高的结构强度、水密封紧密性和低非特异性吸附，这促进了微流控技术在生物医学领域的实际应用。与其他现有的微流控芯片技术相比，我们的纯PP材料微流控芯片及其制造方法具有许多优点，如高速生产率和极低的成本。它可以在工业装配线上实现标准化制造。
