Wang Tian-Xing, Shi Man-Man, Jiang Jian-Guo
College of Food and Bioengineering, South China University of Technology Guangzhou 510640 China
RSC Adv. 2018 Jun 25;8(41):23181-23190. doi: 10.1039/c8ra02803f. eCollection 2018 Jun 21.
(Thunb.) Makino is a medicinal and edible plant in China whose buds and leaves are used for making a popular kind of tea drink. The anticancer and antioxidant properties of the ethyl acetate (EA) and -butanol (-Bu) fractions provide a basis for conducting experiments for isolation and identification of key compounds that may be responsible for the aforementioned properties of . Four compounds were isolated from the two fractions using ODS packing column, silica gel column, polyamide column, Sephadex LH-20 gel column and HPLC. With the aid of H, C NMR and mass spectrometry, they were identified as 3,4-dihydroxy phenyl--β-d-glucoside, gypenoside XLVI, gypenoside L and ginsenoside Rd. 3,4-Dihydroxy phenyl--β-d-glucoside showed the strongest DPPH (97.23%) and ABTS (101.37%) scavenging effect and ferric ion reducing power (FRAP value 0.8846), which may be closely related to the hydrogen atoms of phenolic hydroxyls. Gypenoside L and ginsenoside Rd displayed the highest inhibition of tumor cell proliferation of A549 and MCF-7 cell lines, which had to do with the chemical structure of the compounds bearing glycosylated parts and free hydroxyls at the 20th or 21st carbon atom of dammarane-type saponin.
（Thunb.）Makino是中国一种药食两用植物，其芽和叶用于制作一种受欢迎的茶饮。乙酸乙酯（EA）和正丁醇（-Bu）馏分的抗癌和抗氧化特性为开展实验以分离和鉴定可能导致上述特性的关键化合物提供了依据。使用ODS填充柱、硅胶柱、聚酰胺柱、Sephadex LH - 20凝胶柱和高效液相色谱从这两个馏分中分离出四种化合物。借助氢谱、碳谱核磁共振和质谱，它们被鉴定为3,4 - 二羟基苯基 - β - D - 葡萄糖苷、绞股蓝皂苷XLVI、绞股蓝皂苷L和人参皂苷Rd。3,4 - 二羟基苯基 - β - D - 葡萄糖苷表现出最强的DPPH清除效果（97.23%）和ABTS清除效果（101.37%）以及铁离子还原能力（FRAP值0.8846），这可能与酚羟基的氢原子密切相关。绞股蓝皂苷L和人参皂苷Rd对A549和MCF - 7细胞系的肿瘤细胞增殖具有最高的抑制作用，这与达玛烷型皂苷在第20或21个碳原子上带有糖基化部分和游离羟基的化合物的化学结构有关。
