用于农药检测的适配体传感器。
Aptasensors for pesticide detection.
机构信息
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education (Southeast University), School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education (Southeast University), School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
出版信息
Biosens Bioelectron. 2019 Apr 1;130:174-184. doi: 10.1016/j.bios.2019.01.006. Epub 2019 Jan 15.
Pesticide contamination has become one of the most serious problems of public health in the world, due to their wide application in agriculture industry to guarantee the crop yield and quality. The detection of pesticide residues plays an important role in food safety management and environment protection. However, the conventional detection methodologies cannot realize highly sensitive, selective and on-site detection, which limits their applications. Aptamers are short single-stranded oligonucleotides (RNA or DNA) selected by SELEX method, which can selectively bind to their targets with high affinity. Compared with the commonly used antibodies or enzymes in designing biosensors, aptamers exhibit better stability, low molecular weight, easy modification and low cost, and were regarded as excellent candidates for developing aptasensors for pesticide detection. In this review, application of aptamers for pesticide detection was reviewed. Firstly, aptamers specifically bind to various pesticides were first summarized. Secondly, the progresses and highlights of developing aptasensors for highly-sensitive and selective detection of pesticide residues were systematically provided. Finally, the present challenges and future perspectives for developing novel highly-effective aptasensor for the detection of pesticide residues were discussed.
农药污染已成为世界公共卫生领域最严重的问题之一，这是由于其在农业生产中被广泛应用以保证作物产量和质量。农药残留的检测在食品安全管理和环境保护中起着重要作用。然而，传统的检测方法无法实现高灵敏度、选择性和现场检测，这限制了它们的应用。适配体是通过 SELEX 方法筛选出的短链单链寡核苷酸（RNA 或 DNA），它们可以与高亲和力的目标物选择性结合。与生物传感器设计中常用的抗体或酶相比，适配体具有更好的稳定性、低分子量、易于修饰和低成本的特点，被认为是开发用于农药检测的适配体传感器的理想候选物。本综述主要讨论了适配体在农药检测中的应用。首先，总结了特异性结合各种农药的适配体。其次，系统地提供了用于高灵敏度和选择性检测农药残留的适配体传感器的研究进展和亮点。最后，讨论了开发用于检测农药残留的新型高效适配体传感器所面临的挑战和未来展望。
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