Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö SE-20506, Sweden.
Sensors (Basel). 2017 Mar 21;17(3):645. doi: 10.3390/s17030645.
Rapid and cost-effective analysis of histamine, in food, environmental, and diagnostics research has been of interest recently. However, for certain applications, the already-existing biological receptor-based sensing methods have usage limits in terms of stability and costs. As a result, robust and cost-effective imprinted polymeric receptors can be the best alternative. In the present work, molecularly-imprinted polymers (MIPs) for histamine were synthesized using methacrylic acid in chloroform and acetonitrile as two different porogens. The binding affinity of the MIPs with histamine was evaluated in aqueous media. MIPs synthesized in chloroform displayed better imprinting properties for histamine. We demonstrate here histamine MIPs incorporated into a carbon paste (CP) electrode as a MIP-CP electrode sensor platforms for detection of histamine. This simple sensor format allows accurate determination of histamine in the sub-nanomolar range using an electrochemical method. The sensor exhibited two distinct linear response ranges of 1 × 10-7 × 10 M and 7 × 10-4 × 10 M. The detection limit of the sensor was calculated equal to 7.4 × 10 M. The specificity of the proposed electrode for histamine is demonstrated by using the analogous molecules and other neurotransmitters such as serotonin, dopamine, etc. The MIP sensor was investigated with success on spiked serum samples. The easy preparation, simple procedure, and low production cost make the MIP sensor attractive for selective and sensitive detection of analytes, even in less-equipped laboratories with minimal training.
近年来，快速且经济高效地分析食品、环境和诊断研究中的组氨酸一直是人们关注的焦点。然而，对于某些应用，现有的基于生物受体的传感方法在稳定性和成本方面存在使用限制。因此，稳健且经济高效的印迹聚合物受体可能是最佳选择。在本工作中，使用甲基丙烯酸在氯仿和乙腈中合成了用于组氨酸的分子印迹聚合物（MIPs），这两种溶剂分别作为两种不同的致孔剂。在水介质中评估了 MIPs 与组氨酸的结合亲和力。在氯仿中合成的 MIPs 对组氨酸表现出更好的印迹特性。我们在这里展示了将组氨酸 MIPs 掺入到碳糊（CP）电极中作为 MIP-CP 电极传感器平台，用于检测组氨酸。这种简单的传感器格式允许使用电化学方法在亚纳摩尔范围内准确测定组氨酸。传感器显示出两个明显的线性响应范围，分别为 1×10-7×10 M 和 7×10-4×10 M。传感器的检测限计算为 7.4×10 M。通过使用类似的分子和其他神经递质（如 5-羟色胺、多巴胺等）证明了所提出的电极对组氨酸的特异性。该 MIP 传感器已成功用于加标血清样品的研究。该 MIP 传感器具有易于制备、简单的操作程序和低制造成本等优点，即使在配备最少训练设备的欠发达实验室中，也具有对分析物进行选择性和灵敏检测的吸引力。
