Wang Gang, Zhu Jiehua, Chen Xiaofeng, Dong Hua, Li Qingtao, Zeng Lei, Cao Xiaodong
Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology Guangzhou 510641 PR China
National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou 510006 PR China.
RSC Adv. 2018 Mar 21;8(20):11036-11042. doi: 10.1039/c8ra00668g. eCollection 2018 Mar 16.
In recent years medical devices manufacturers have been looking for antimicrobial coatings which are biocompatible and non-toxic for a wide range of medical devices. The demand for these antimicrobial coatings has increased significantly, owing to the increased incidence of hospital-associated infections (HAIs). Hydrogels have been widely used in biomedical applications due to their hydrophilicity, biodegradability, non-toxicity and biocompatibility. In this work, sodium alginate (SA) based antibacterial hydrogels SA/PEG-HHC10 were designed and prepared by combining Diels-Alder (DA) click chemistry and the thiol-ene reaction. The hydrogels were first prepared using DA click chemistry with good mechanical strength, then the cysteine-terminated antimicrobial peptide HHC10-CYS (HHC10) was grafted into the hydrogel by the thiol-ene reaction between the oxy-norbornene group and the thiol group. The results showed that the antimicrobial hydrogels had a strong antibacterial property and good biocompatibility. Therefore, the antimicrobial hydrogels have significant potential application as coatings for implantable medical devices.
近年来，医疗设备制造商一直在寻找适用于各种医疗设备的具有生物相容性且无毒的抗菌涂层。由于医院获得性感染（HAIs）的发生率增加，对这些抗菌涂层的需求显著上升。水凝胶因其亲水性、生物可降解性、无毒和生物相容性而被广泛应用于生物医学领域。在这项工作中，通过结合狄尔斯-阿尔德（DA）点击化学和硫醇-烯反应，设计并制备了基于海藻酸钠（SA）的抗菌水凝胶SA/PEG-HHC10。首先利用DA点击化学制备具有良好机械强度的水凝胶，然后通过氧降冰片烯基团与硫醇基团之间的硫醇-烯反应将半胱氨酸末端抗菌肽HHC10-CYS（HHC10）接枝到水凝胶中。结果表明，该抗菌水凝胶具有很强的抗菌性能和良好的生物相容性。因此，这种抗菌水凝胶作为可植入医疗设备的涂层具有显著的潜在应用价值。
