Clinical Research Center, Masan National Tuberculosis Hospital, Changwon, Republic of Korea.
Amicogen Inc., Jinju-si, Republic of Korea.
J Appl Microbiol. 2022 Aug;133(2):1089-1098. doi: 10.1111/jam.15618. Epub 2022 May 31.
The discovery of antiviral substances to respond to COVID-19 is a global issue, including the field of drug development based on natural materials. Here, we showed that chitosan-based substances have natural antiviral properties against SARS-CoV-2 in vitro.
The molecular weight of chitosan-based substances was measured by the gel permeation chromatography analysis. In MTT assay, the chitosan-based substances have low cytotoxicity to Vero cells. The antiviral effect of these substances was confirmed by quantitative viral RNA targeting the RdRp and E genes and plaque assay. Among the substances tested, low molecular weight chitooligosaccharide decreased the fluorescence intensity of SARS-CoV-2 nucleocapsid protein of the virus-infected cells in a dose-dependent manner.
In conclusion, the chitooligosaccharide, a candidate for natural treatment, has antiviral effects against the SARS-CoV-2 virus in vitro.
In this study, it was suggested for the first time that chitosan-based substances such as chitooligosaccharide can have an antiviral effect on SARS-CoV-2 in vitro.
应对 COVID-19 的抗病毒物质的发现是一个全球性问题，包括基于天然材料的药物开发领域。在这里，我们表明壳聚糖基物质在体外对 SARS-CoV-2 具有天然的抗病毒特性。
通过凝胶渗透色谱分析测量了壳聚糖基物质的分子量。在 MTT 测定中，壳聚糖基物质对 Vero 细胞的细胞毒性低。通过定量针对 RdRp 和 E 基因的病毒 RNA 和噬斑测定证实了这些物质的抗病毒作用。在所测试的物质中，低分子量壳寡糖以剂量依赖的方式降低了感染病毒的细胞中 SARS-CoV-2 核衣壳蛋白的荧光强度。
总之，壳寡糖作为天然治疗候选物，在体外对 SARS-CoV-2 病毒具有抗病毒作用。
在这项研究中，首次表明壳聚糖基物质，如壳寡糖，在体外对 SARS-CoV-2 具有抗病毒作用。
