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四种药用植物叶绿体基因组的比较分析

A Comparative Analysis of the Chloroplast Genomes of Four Medicinal Plants.

作者信息

Guo Shuai, Liao Xuejiao, Chen Shiyu, Liao Baosheng, Guo Yiming, Cheng Ruiyang, Xiao Shuiming, Hu Haoyu, Chen Jun, Pei Jin, Chen Yangjin, Xu Jiang, Chen Shilin

机构信息

Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

出版信息

Front Genet. 2022 Apr 25;13:764534. doi: 10.3389/fgene.2022.764534. eCollection 2022.

Abstract

is a generalized genus of the Polygonaceae family that includes various herbaceous plants. In order to provide aid in understanding the evolutionary and phylogenetic relationship in at the chloroplast (cp) genome-scale level, we sequenced and annotated the complete chloroplast genomes of four species using next-generation sequencing technology and CpGAVAS. Then, repeat sequences, IR contractions, and expansion and transformation sites of chloroplast genomes of four species were studied, and a phylogenetic tree was built using the chloroplast genomes of . The results indicated that the chloroplast genome construction of also displayed characteristic four types of results, comparable to the published chloroplast genome of recorded angiosperms. The chloroplast genomes of the four plants are highly consistent in genome size (159,015 bp-163,461 bp), number of genes (112 genes, including 78 protein-coding genes, 30 tRNA genes, and 4 rRNA genes), gene types, gene order, codon usage, and repeat sequence distribution, which identifies the high preservation among the chloroplast genomes. The phylogenetic tree was recreated by a full sequence of the chloroplast genome, which illustrates that the , , and are divided into the same branch, and belongs to . The precise system site of lots base parts requires further verification, but the study would provide a basis for developing the available genetic resources and evolutionary relationships of .

摘要

是蓼科的一个广义属,包括各种草本植物。为了在叶绿体(cp)基因组规模水平上帮助理解其进化和系统发育关系,我们使用下一代测序技术和CpGAVAS对四个物种的完整叶绿体基因组进行了测序和注释。然后,研究了四个物种叶绿体基因组的重复序列、IR收缩以及扩增和转化位点,并使用叶绿体基因组构建了系统发育树。结果表明,其叶绿体基因组结构也呈现出与已发表的有记录被子植物叶绿体基因组相当的四种特征类型。这四种植物的叶绿体基因组在基因组大小(159,015 bp - 163,461 bp)、基因数量(112个基因,包括78个蛋白质编码基因、30个tRNA基因和4个rRNA基因)、基因类型、基因顺序、密码子使用和重复序列分布方面高度一致,这表明该属叶绿体基因组具有高度保守性。通过叶绿体基因组的全序列重建了系统发育树,结果表明,[物种名称1]、[物种名称2]和[物种名称3]被分为同一分支,而[物种名称4]属于[另一分支名称]。许多碱基部分的确切系统位置需要进一步验证,但该研究将为开发该属可用的遗传资源和进化关系提供依据。

需注意,原文中部分物种名称缺失,以上翻译是在假设补充完整物种名称后的完整译文。实际翻译时需根据准确的物种名称进行调整。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eec/9084321/517211ad84bc/fgene-13-764534-g001.jpg

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