金纳米颗粒及金纳米颗粒标记抗体作为共反应物的电化学发光

Zhao Changzhi, Niu Lulu, Wang Xiaoyu, Sun Wei

Key Laboratory of Sensor Analysis of Tumor Marker, The Ministry of Education, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Qingdao 266042 China

Key Laboratory of Functional Materials and Photoelectronchemistry of Haikou, College of Chemistry and Chemical Engineering, Hainan Normal University Haikou 571158 China

RSC Adv. 2018 Oct 24;8(63):36219-36222. doi: 10.1039/c8ra07429a. eCollection 2018 Oct 22.

Gold nanoparticles (AuNPs) and AuNP-labelled antibodies can participate in the electrochemiluminescence (ECL) reaction of tris(2,2'-bipyridyl) ruthenium(ii) (Ru(bpy) ) as a co-reactant. The electrochemical and spectral characteristics of this new ECL system were confirmed by experiments, and its reaction mechanism was speculated to be different from that of typical ECL. The experimental results showed that the new ECL system exhibited higher ECL efficiency, and AuNP-labelled substances can be sensitively measured with the help of AuNPs. Based on the dual role of AuNPs as a co-reactant and marker, the AuNP-Ru(bpy) ECL system will be a useful tool in biochemical analysis.

金纳米颗粒（AuNPs）和AuNP标记的抗体可以作为共反应物参与三（2,2'-联吡啶）钌（II）（Ru(bpy) ）的电化学发光（ECL）反应。通过实验证实了这种新型ECL体系的电化学和光谱特性，并推测其反应机理与典型的ECL不同。实验结果表明，新型ECL体系具有更高的ECL效率，并且借助AuNPs可以灵敏地检测AuNP标记的物质。基于AuNPs作为共反应物和标记物的双重作用，AuNP-Ru(bpy) ECL体系将成为生化分析中的一种有用工具。