• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

双阳离子两亲分子稳定的金纳米颗粒的表面疏水性对A549肺癌细胞的作用

Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized Gold Nanoparticles on A549 Lung Cancer Cells.

作者信息

Muthukumarasamyvel Thangavel, Rajendran Ganapathy, Santhana Panneer Devendrapandi, Kasthuri Jayapalan, Kathiravan Krishnan, Rajendiran Nagappan

机构信息

Department of Polymer Science and Department of Biotechnology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.

Department of Chemistry, Quaid-E-Millath Government College for Women (Autonomous), Chennai 600002, Tamil Nadu, India.

出版信息

ACS Omega. 2017 Jul 31;2(7):3527-3538. doi: 10.1021/acsomega.7b00353. Epub 2017 Jul 25.

DOI:10.1021/acsomega.7b00353
PMID:30023697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6044882/
Abstract

Herein, we report the surface functionality of dicationic cysteamine conjugated cholic acid (DCaC), dicationic cysteamine conjugated deoxycholic acid (DCaDC), and dicationic cysteamine conjugated lithocholic acid (DCaLC) templated gold nanoparticles (AuNPs) on mammalian cells. The haemocompatibility of the synthesized NPs was evaluated by in vitro hemolysis and erythrocyte sedimentation rate using human red blood cells (RBCs). In all of the systems, no toxicity was observed on human erythrocytes (RBCs) up to the concentration of 120 μg/mL. The anticancer activity of these dicationic amphiphile-stabilized AuNPs on A549 lung cancer cells was demonstrated by in vitro cell viability assay, intracellular reactive oxygen species estimation by DCFH-DA, apoptosis analysis using AO-EtBr fluorescence staining, DNA fragmentation analysis by agarose gel electrophoresis, and western blot analysis of caspase-3 expression. These results suggest that the cytotoxicity of AuNPs to A549 cells increase with the dose and hydrophobicity of amphiphiles and were found to be in the order: DCaLC-AuNPs > DCaDC-AuNPs > DCaC-AuNPs.

摘要

在此，我们报告了二价半胱胺共轭胆酸（DCaC）、二价半胱胺共轭脱氧胆酸（DCaDC）和二价半胱胺共轭石胆酸（DCaLC）模板化金纳米颗粒（AuNPs）在哺乳动物细胞上的表面功能。使用人红细胞（RBCs）通过体外溶血和红细胞沉降率评估合成纳米颗粒的血液相容性。在所有系统中，在浓度高达120μg/mL时，未观察到对人红细胞（RBCs）的毒性。通过体外细胞活力测定、DCFH-DA法测定细胞内活性氧、AO-EtBr荧光染色法进行凋亡分析、琼脂糖凝胶电泳法进行DNA片段分析以及caspase-3表达的蛋白质印迹分析，证明了这些二价两亲物稳定的AuNPs对A549肺癌细胞的抗癌活性。这些结果表明，AuNPs对A549细胞的细胞毒性随着两亲物的剂量和疏水性增加而增加，并且发现顺序为：DCaLC-AuNPs > DCaDC-AuNPs > DCaC-AuNPs。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/8cd8b1e33a35/ao-2017-00353x_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/3f7f4ff36ad8/ao-2017-00353x_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/6445151a813e/ao-2017-00353x_0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/d08b5966e67e/ao-2017-00353x_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/69cf52486da7/ao-2017-00353x_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/df91129d7012/ao-2017-00353x_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/aee2225856bb/ao-2017-00353x_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/a21d29f11e13/ao-2017-00353x_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/bb0ce4b8a527/ao-2017-00353x_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/49c82ebcf28b/ao-2017-00353x_0012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/3ff3915e1a56/ao-2017-00353x_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/87de9012e0c0/ao-2017-00353x_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/8cd8b1e33a35/ao-2017-00353x_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/3f7f4ff36ad8/ao-2017-00353x_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/6445151a813e/ao-2017-00353x_0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/d08b5966e67e/ao-2017-00353x_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/69cf52486da7/ao-2017-00353x_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/df91129d7012/ao-2017-00353x_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/aee2225856bb/ao-2017-00353x_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/a21d29f11e13/ao-2017-00353x_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/bb0ce4b8a527/ao-2017-00353x_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/49c82ebcf28b/ao-2017-00353x_0012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/3ff3915e1a56/ao-2017-00353x_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/87de9012e0c0/ao-2017-00353x_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899b/6643781/8cd8b1e33a35/ao-2017-00353x_0010.jpg

相似文献

1
Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized Gold Nanoparticles on A549 Lung Cancer Cells.双阳离子两亲分子稳定的金纳米颗粒的表面疏水性对A549肺癌细胞的作用
ACS Omega. 2017 Jul 31;2(7):3527-3538. doi: 10.1021/acsomega.7b00353. Epub 2017 Jul 25.
2
Auric Chloride Induced Micellization on Fractal Patterned Dicationic Amphiphiles and Stabilization of Gold Nanoparticles.氯化金在分形图案化双阳离子两亲物上诱导的胶束化及金纳米颗粒的稳定化
ACS Omega. 2017 Jul 25;2(7):3539-3550. doi: 10.1021/acsomega.7b00192. eCollection 2017 Jul 31.
3
Hierarchical Self-Assembly of Bile-Acid-Derived Dicationic Amphiphiles and Their Toxicity Assessment on Microbial and Mammalian Systems.基于胆汁酸衍生的双阳离子两亲分子的分级自组装及其对微生物和哺乳动物系统的毒性评估。
ACS Appl Mater Interfaces. 2016 Sep 28;8(38):25111-26. doi: 10.1021/acsami.6b08018. Epub 2016 Sep 13.
4
Biosynthesis of gold nanoparticles using Vetex negundo and evaluation of pro-apoptotic effect on human gastric cancer cell lines.利用荆芥合成金纳米粒子及其对人胃癌细胞系促凋亡作用的评价。
J Photochem Photobiol B. 2020 Jan;203:111749. doi: 10.1016/j.jphotobiol.2019.111749. Epub 2019 Dec 17.
5
Photosynthesized gold nanoparticles from Catharanthus roseus induces caspase-mediated apoptosis in cervical cancer cells (HeLa).从长春花中光合作用合成的金纳米粒子诱导宫颈癌（HeLa）细胞发生 caspase 介导线粒体凋亡。
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1938-1946. doi: 10.1080/21691401.2019.1614017.
6
Selenium-Containing Amphiphiles Reduced and Stabilized Gold Nanoparticles: Kill Cancer Cells via Reactive Oxygen Species.含硒两亲分子还原并稳定金纳米粒子：通过活性氧物种杀死癌细胞。
ACS Appl Mater Interfaces. 2016 Aug 31;8(34):22106-12. doi: 10.1021/acsami.6b08282. Epub 2016 Aug 19.
7
Gold nanoparticles-conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt-mediated pathway in breast cancer cell lines (MCF-7 and MDA-MB-231).金纳米颗粒偶联槲皮素通过抑制表皮生长因子受体/磷脂酰肌醇-3激酶/蛋白激酶B介导的信号通路诱导乳腺癌细胞系（MCF-7和MDA-MB-231）凋亡。
Cell Biochem Funct. 2017 Jun;35(4):217-231. doi: 10.1002/cbf.3266. Epub 2017 May 12.
8
Anticancer activity of green synthesised gold nanoparticles from inhibits A549 cell proliferation through the apoptotic pathway.从 中绿色合成的金纳米粒子的抗癌活性通过凋亡途径抑制 A549 细胞增殖。
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):4012-4019. doi: 10.1080/21691401.2019.1575844.
9
Therapeutic effects of gold nanoparticles synthesized using Musa paradisiaca peel extract against multiple antibiotic resistant Enterococcus faecalis biofilms and human lung cancer cells (A549).利用芭蕉果皮提取物合成的金纳米颗粒对多重耐药粪肠球菌生物膜和人肺癌细胞（A549）的治疗作用
Microb Pathog. 2017 Jan;102:173-183. doi: 10.1016/j.micpath.2016.11.029. Epub 2016 Dec 1.
10
Biosynthesis of gold nanoparticles and related cytotoxicity evaluation using A549 cells.金纳米粒子的生物合成及其对 A549 细胞的相关细胞毒性评价。
Ecotoxicol Environ Saf. 2015 Apr;114:232-40. doi: 10.1016/j.ecoenv.2014.03.020. Epub 2014 May 14.

引用本文的文献

1
pH-responsive nanocapsules loaded with 5-fluorouracil-coated green-synthesized CuO-ZnO NPs for enhanced anticancer activity against HeLa cells.负载5-氟尿嘧啶的pH响应性纳米胶囊包裹绿色合成的CuO-ZnO纳米颗粒，用于增强对HeLa细胞的抗癌活性。
Med Oncol. 2025 Jun 30;42(8):300. doi: 10.1007/s12032-025-02847-6.
2
Folate-Functionalized CS/rGO/NiO Nanocomposites as a Multifunctional Drug Carrier with Anti-Microbial, Target-Specific, and Stimuli-Responsive Capacities.叶酸功能化的壳聚糖/还原氧化石墨烯/氧化镍纳米复合材料作为一种具有抗菌、靶向特异性和刺激响应能力的多功能药物载体。
Int J Nanomedicine. 2025 Feb 14;20:1965-1981. doi: 10.2147/IJN.S489418. eCollection 2025.
3

本文引用的文献

1
Anticancer activity of eco-friendly gold nanoparticles against lung and liver cancer cells.环保型金纳米颗粒对肺癌和肝癌细胞的抗癌活性。
J Genet Eng Biotechnol. 2016 Jun;14(1):195-202. doi: 10.1016/j.jgeb.2016.05.007. Epub 2016 Jun 9.
2
Linn: Biogenic synthesis of silver and gold nanoparticles and their cytotoxic effects against MDA-MB-231, human breast cancer cells.林恩：银和金纳米颗粒的生物合成及其对人乳腺癌细胞MDA-MB-231的细胞毒性作用。
Biotechnol Rep (Amst). 2014 Aug 13;4:42-49. doi: 10.1016/j.btre.2014.08.002. eCollection 2014 Dec.
3
Hierarchical Self-Assembly of Bile-Acid-Derived Dicationic Amphiphiles and Their Toxicity Assessment on Microbial and Mammalian Systems.
The toxicity of nanoparticles and their interaction with cells: an metabolomic perspective.
纳米颗粒的毒性及其与细胞的相互作用：代谢组学视角
Nanoscale Adv. 2023 Jan 30;5(10):2674-2723. doi: 10.1039/d2na00534d. eCollection 2023 May 16.
4
Gold Nanoparticles Induced Size Dependent Cytotoxicity on Human Alveolar Adenocarcinoma Cells by Inhibiting the Ubiquitin Proteasome System.金纳米颗粒通过抑制泛素蛋白酶体系统对人肺泡腺癌细胞产生大小依赖性细胞毒性。
Pharmaceutics. 2023 Jan 28;15(2):432. doi: 10.3390/pharmaceutics15020432.
5
Electrochemiluminescence of gold nanoparticles and gold nanoparticle-labelled antibodies as co-reactants.金纳米颗粒及金纳米颗粒标记抗体作为共反应物的电化学发光
RSC Adv. 2018 Oct 24;8(63):36219-36222. doi: 10.1039/c8ra07429a. eCollection 2018 Oct 22.
6
Immune cell engineering: opportunities in lung cancer therapeutics.免疫细胞工程：肺癌治疗中的机遇。
Drug Deliv Transl Res. 2020 Oct;10(5):1203-1227. doi: 10.1007/s13346-020-00719-2.
7
The Rational Design and Biological Mechanisms of Nanoradiosensitizers.纳米放射增敏剂的合理设计与生物学机制
Nanomaterials (Basel). 2020 Mar 11;10(3):504. doi: 10.3390/nano10030504.
8
Cytotoxicity-Related Bioeffects Induced by Nanoparticles: The Role of Surface Chemistry.纳米颗粒诱导的细胞毒性相关生物效应：表面化学的作用
Front Bioeng Biotechnol. 2019 Dec 12;7:414. doi: 10.3389/fbioe.2019.00414. eCollection 2019.
9
Method development for optimised green synthesis of gold nanoparticles from and their activity in non-small cell lung cancer cell lines.开发从 中优化绿色合成金纳米粒子的方法及其在非小细胞肺癌细胞系中的活性。
IET Nanobiotechnol. 2019 Aug;13(6):626-633. doi: 10.1049/iet-nbt.2018.5410.
10
Preparation and In Vitro Release of Total Alkaloids from Leaves Loaded mPEG-PLA Microspheres.载甲氧基聚乙二醇-聚乳酸微球叶片中总生物碱的制备及体外释放
Materials (Basel). 2019 May 6;12(9):1457. doi: 10.3390/ma12091457.
基于胆汁酸衍生的双阳离子两亲分子的分级自组装及其对微生物和哺乳动物系统的毒性评估。
ACS Appl Mater Interfaces. 2016 Sep 28;8(38):25111-26. doi: 10.1021/acsami.6b08018. Epub 2016 Sep 13.
4
Selenium-Containing Amphiphiles Reduced and Stabilized Gold Nanoparticles: Kill Cancer Cells via Reactive Oxygen Species.含硒两亲分子还原并稳定金纳米粒子：通过活性氧物种杀死癌细胞。
ACS Appl Mater Interfaces. 2016 Aug 31;8(34):22106-12. doi: 10.1021/acsami.6b08282. Epub 2016 Aug 19.
5
Tuning the anticancer activity of a novel pro-apoptotic peptide using gold nanoparticle platforms.利用金纳米颗粒平台调控一种新型促凋亡肽的抗癌活性。
Sci Rep. 2016 Aug 4;6:31030. doi: 10.1038/srep31030.
6
Polyphenol stabilized colloidal gold nanoparticles from Abutilon indicum leaf extract induce apoptosis in HT-29 colon cancer cells.锦葵叶多酚稳定的胶体金纳米粒子诱导 HT-29 结肠癌细胞凋亡。
Colloids Surf B Biointerfaces. 2016 Jul 1;143:499-510. doi: 10.1016/j.colsurfb.2016.03.069. Epub 2016 Mar 26.
7
In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina nudiflora L. Aqueous Extract Against HCT-116 Colon Cancer Cells.利用鸭跖草水提取物合成的金、银纳米粒子对HCT-116结肠癌细胞的体外抗癌活性
Biol Trace Elem Res. 2016 Oct;173(2):297-305. doi: 10.1007/s12011-016-0666-7. Epub 2016 Mar 9.
8
Nepenthes khasiana mediated synthesis of stabilized gold nanoparticles: Characterization and biocompatibility studies.卡西猪笼草介导的稳定金纳米颗粒的合成：表征与生物相容性研究
J Photochem Photobiol B. 2016 Jan;154:108-17. doi: 10.1016/j.jphotobiol.2015.12.002. Epub 2015 Dec 9.
9
Deposition of superparamagnetic nanohydroxyapatite on iron-fibrin substrates: preparation, characterization, cytocompatibility and bioactivity studies.超顺磁性纳米羟基磷灰石在铁纤维蛋白基质上的沉积：制备、表征、细胞相容性及生物活性研究
Colloids Surf B Biointerfaces. 2014 Aug 1;120:208-14. doi: 10.1016/j.colsurfb.2014.04.021. Epub 2014 May 2.
10
Protein coronas suppress the hemolytic activity of hydrophilic and hydrophobic nanoparticles.蛋白质冠层可抑制亲水性和疏水性纳米颗粒的溶血活性。
Mater Horiz. 2014 Jan 1;1(1):102-105. doi: 10.1039/C3MH00075C.