Doctoral School, University of Silesia, Bankowa 14, 40-032 Katowice, Poland.
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland.
Int J Mol Sci. 2022 Apr 29;23(9):4966. doi: 10.3390/ijms23094966.
Although the molecular response of bacteria exposed to metal nanoparticles (NPs) is intensively studied, many phenomena related to their survival, metal uptake, gene expression and protein production are not fully understood. Therefore, this work aimed to study Ag-NPs, Cu-NPs, ZnO-NPs and TiO-NPs-induced alterations in the expression level of selected oxidative stress-related genes in connection with the activity of antioxidant enzymes: catalase (CAT), peroxidase (PER) and superoxide dismutase (SOD) in , and . The methodology used included: the extraction of total RNA and cDNA synthesis, the preparation of primers for selected housekeeping and oxidative stress genes, RT-qPCR reaction and the measurements of CAT, PER and SOD activities. It was established that the treatment of and with NPs resulted mainly in the down-regulation of targeted genes, whilst the up-regulation of genes was confirmed in . The greatest differences in the relative expression levels of tested genes occurred in and treated with TiO-NPs, while in , they were observed under ZnO-NPs exposure. The changes found were mostly related to the expression of genes encoding proteins with PER and CAT-like activity. Among NPs, ZnO-NPs and Cu-NPs increased the activity of antioxidants in and . In turn, TiO-NPs had a major effect on enzymes activity in . Considering all of the collected results for tested bacteria, it can be emphasised that the impact of NPs on the antioxidant system functioning was dependent on their type and concentration.
尽管细菌暴露于金属纳米颗粒（NPs）时的分子反应受到了广泛研究，但许多与它们的生存、金属摄取、基因表达和蛋白质产生相关的现象仍未被充分理解。因此，本工作旨在研究 Ag-NPs、Cu-NPs、ZnO-NPs 和 TiO-NPs 诱导 、 和 中与抗氧化酶活性相关的选定氧化应激相关基因表达水平的变化：过氧化氢酶（CAT）、过氧化物酶（PER）和超氧化物歧化酶（SOD）。所采用的方法包括：总 RNA 的提取和 cDNA 合成、选定管家基因和氧化应激基因的引物制备、RT-qPCR 反应以及 CAT、PER 和 SOD 活性的测量。结果表明，NPs 主要导致 和 中靶基因下调，而 中证实了基因的上调。在 TiO-NPs 处理的 和 中，测试基因的相对表达水平的差异最大，而在 ZnO-NPs 暴露下，差异最大的是 。发现的变化主要与具有 PER 和 CAT 样活性的蛋白编码基因的表达有关。在 NPs 中，ZnO-NPs 和 Cu-NPs 增加了 和 中抗氧化剂的活性。反过来，TiO-NPs 对 的酶活性有重大影响。考虑到所有测试细菌的收集结果，可以强调 NPs 对抗氧化系统功能的影响取决于它们的类型和浓度。
