Shao Li, Huang Jieyan, Li Yan, Ma Laiji, Niu Yujie, Jiang Wen, Yuan Chunying, Bai Tianming, Yang Suzhen
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, P. R. China.
R&D Innovation Center, Shandong Freda Biotech Co., Ltd., Jinan, Shandong, P. R. China.
J Cosmet Dermatol. 2025 Mar;24(3):e70105. doi: 10.1111/jocd.70105.
Oxidative stress activates the reactive oxygen species (ROS) and excessive ROS can damage skin cells, initiating oxidative stress responses that contribute to inflammation, aging, and other skin issues. As a resident skin bacterium, Cutibacterium acnes (C. acnes) plays an important role in maintaining skin homeostasis and provides antioxidant benefits. However, the metabolite components and mechanisms of C. acnes exerting antioxidant activity are not yet clear. This study aimed to analyze the potential antioxidant effects of C. acnes cell-free supernatant and the mechanisms.
The antioxidant effects were evaluated by measuring the scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt) (ABTS) radicals, and hydroxyl radicals, as well as the effects on ROS levels in menadione-induced primary human keratinocytes in vitro. Additionally, western blot analysis was performed to assess the antioxidant effects of the C. acnes CCSM0331 cell-free supernatant (CFS).
C. acnes CCSM0331 was isolated from the facial skin of healthy individuals. This strain, classified as type II, is associated with healthy skin. The CFS of strain CCSM0331 contained various short-chain fatty acids (SCFAs), glutathione peroxidase (GSH-Px), and total superoxide dismutase(T-SOD), exhibiting strong DPPH and ABTS radical scavenging capabilities, thus demonstrating substantial antioxidant activity. In a reactive oxygen species model induced by menadione in primary human keratinocytes, the addition of 5% of the fermentation supernatant from this strain significantly reduced ROS levels, indicating a notable ROS-scavenging effect. Western blot analysis further confirmed that the CCSM0331 fermentation supernatant activated the expression of Nrf-2 and HO-1 proteins, thereby activating the Nrf-2 oxidative stress pathway and exerting antioxidant effects.
C. acnes CCSM0331 is a promising skin probiotic with notable antioxidant properties. The activity of this strain exhibited significant free radical scavenging activity, suggesting its potential application in the development of antiaging products. This study provides theoretical support for the screening of functional skin bacteria or skin probiotics.
氧化应激会激活活性氧（ROS），过量的ROS会损害皮肤细胞，引发氧化应激反应，进而导致炎症、衰老和其他皮肤问题。痤疮丙酸杆菌（C. acnes）作为一种常驻皮肤细菌，在维持皮肤稳态方面发挥着重要作用，并具有抗氧化益处。然而，痤疮丙酸杆菌发挥抗氧化活性的代谢物成分及机制尚不清楚。本研究旨在分析痤疮丙酸杆菌无细胞上清液的潜在抗氧化作用及其机制。
通过测量2,2-二苯基-1-苦基肼（DPPH）、2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸铵盐）（ABTS）自由基以及羟自由基的清除活性，以及对体外甲萘醌诱导的原代人角质形成细胞中ROS水平的影响，来评估抗氧化作用。此外，进行蛋白质免疫印迹分析以评估痤疮丙酸杆菌CCSM0331无细胞上清液（CFS）的抗氧化作用。
痤疮丙酸杆菌CCSM0331是从健康个体的面部皮肤中分离出来的。该菌株被归类为II型，与健康皮肤相关。CCSM0331菌株的CFS含有多种短链脂肪酸（SCFAs）、谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD），表现出很强的DPPH和ABTS自由基清除能力，从而证明了其显著的抗氧化活性。在原代人角质形成细胞由甲萘醌诱导的活性氧模型中，添加该菌株5%的发酵上清液可显著降低ROS水平，表明其具有显著的ROS清除作用。蛋白质免疫印迹分析进一步证实，CCSM0331发酵上清液激活了Nrf-2和HO-1蛋白的表达，从而激活Nrf-2氧化应激途径并发挥抗氧化作用。
痤疮丙酸杆菌CCSM0331是一种具有显著抗氧化特性的有前景的皮肤益生菌。该菌株的活性表现出显著的自由基清除活性，表明其在抗老化产品开发中的潜在应用。本研究为功能性皮肤细菌或皮肤益生菌的筛选提供了理论支持。
