Zandoná Meleiro Mônica Cristina, de Carvalho Hianka Jasmyne Costa, Ribeiro Rafaela Rodrigues, da Silva Mônica Duarte, Salles Gomes Cristina Massoco, Miglino Maria Angélica, de Santis Prada Irvênia Luiza
Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.
Animals (Basel). 2022 May 7;12(9):1203. doi: 10.3390/ani12091203.
Racehorses are constantly exposed to stress. Aiming to verify the state of blood components and cortisol alterations during their routine and after races, phagocytosis and oxidative neutrophil burst assays, serum cortisol determination, erythrocytes apoptosis evaluation, lymphoproliferation assays, and blood count tests were performed in thirty Thoroughbred racehorses, which were divided in two groups. The samples were taken right after races (moment 0 d), during rest periods (-11 d, +1 d, +3 d), and after training (-8, +2, +5). In both groups, the phagocytosis showed a decrease in percentage and intensity immediately after the race when comparing samples collected during rest or training periods. In the mean values of oxidative burst on samples collected immediately after the race, group I animals demonstrated a decrease (524.2 ± 248.9) when compared with those samples collected in other moments. No significant differences were found between the results of different moments regarding the apoptotic cells and lymphoproliferation assays. The mean values of serum cortisol levels were increased immediately after racing. There was an increase in the percentage of neutrophils found immediately after the race. It was possible to conclude that, although a transient reduction was found in the number of neutrophils, the horses' adaptive function was not affected.
赛马经常面临压力。为了验证赛马日常训练期间及赛后血液成分状态和皮质醇变化情况，对30匹纯种赛马进行了吞噬作用和中性粒细胞氧化爆发试验、血清皮质醇测定、红细胞凋亡评估、淋巴细胞增殖试验以及血细胞计数测试，这些赛马被分为两组。样本在赛后即刻（0天时刻）、休息期（-11天、+1天、+3天）以及训练后（-8天、+2天、+5天）采集。在两组中，与休息或训练期间采集的样本相比，赛后即刻吞噬作用的百分比和强度均降低。在赛后即刻采集的样本中，I组动物的氧化爆发平均值（524.2±248.9）与其他时刻采集的样本相比有所降低。在不同时刻的凋亡细胞和淋巴细胞增殖试验结果之间未发现显著差异。赛后血清皮质醇水平平均值即刻升高。赛后即刻发现中性粒细胞百分比增加。可以得出结论，尽管中性粒细胞数量出现短暂减少，但马匹的适应功能未受影响。
