The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9PS, UK.
Sci Rep. 2022 Jan 10;12(1):427. doi: 10.1038/s41598-021-04137-3.
Neutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. Our proposed study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program. The objectives of this study are to characterise the effect of training on the local pulmonary immune system by defining the gene and protein expression of tracheal wash (TW) derived samples from Thoroughbred racehorses prior to and following commencement of race training. Multiomics analysis detected 2138 differentially expressed genes and 260 proteins during the training period. Gene and protein sets were enriched for biological processes related to acute phase response, oxidative stress, haemopoietic processes, as well as to immune response and inflammation. This study demonstrated TW samples to represent a rich source of airway cells, protein and RNA to study airway immunity in the horse and highlighted the benefits of a multiomics methodological approach to studying the dynamics of equine airway immunity. Findings likely reflect the known associations between race-training and both airway inflammation and bleeding, offering further insight into the potential mechanisms which underpin training associated airway inflammation.
中性粒细胞性气道炎症在训练中的赛马中极为普遍，大多数此类病例都被称为轻度至中度马哮喘（MMEA）。我们的研究主要来源于赛马中的 MMEA 与他们进入赛马训练计划之间的强烈关联。本研究的目的是通过定义在开始赛马训练之前和之后从纯种赛马的气管冲洗（TW）中提取的样本的基因和蛋白质表达，来描述训练对局部肺免疫系统的影响。在训练期间，多组学分析检测到 2138 个差异表达基因和 260 种蛋白质。基因和蛋白质集富集了与急性期反应、氧化应激、造血过程以及免疫反应和炎症相关的生物学过程。本研究表明，TW 样本代表了气道细胞、蛋白质和 RNA 的丰富来源，可用于研究马的气道免疫，并强调了采用多组学方法研究马气道免疫动态的益处。研究结果可能反映了众所周知的训练与气道炎症和出血之间的关联，为支持与训练相关的气道炎症的潜在机制提供了进一步的见解。
