National and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
School of Traditional Chinese Material Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Molecules. 2022 Apr 20;27(9):2632. doi: 10.3390/molecules27092632.
The excessive expression of reactive oxygen species is closely connected to many diseases. Considerable studies have demonstrated dandelion as well as its ingredients exhibited antioxidant activity. However, specific material basis reflecting the antioxidant activity has not been comprehensively investigated. In this study, a spectrum-effect relationship study on dandelion between fingerprinting and antioxidant activity was analyzed in detail, while a UHPLC quantification method developed and completely validated for simultaneous determination of active ingredients in dandelion. With the establishment of dandelion fingerprints of different regions, 24 common peaks were characterized. The classic FRAP method and ABTS methods were then used to detect their antioxidant activity. Partial least squares regression analysis, bivariate correlation analysis and grey correlation method were used to accomplish the spectrum-effect relationship. Eventually, the ingredients with antioxidant activity which could be considered as candidate quality markers of dandelion were discovered through spectrum-effect relationship analysis. The six compounds including caftaric acid, chlorogenic acid, caffeic acid, chicoric acid, isochlorogenic acid A, and isochlorogenic acid C were quantitatively determined. The developed UHPLC assay method was accurate, precise, and reliable. The study has elucidated the antioxidant material basis of dandelion and provided a scientific basis for the quality control of dandelion.
活性氧的过度表达与许多疾病密切相关。大量研究表明，蒲公英及其成分具有抗氧化活性。然而，反映抗氧化活性的具体物质基础尚未得到全面研究。在这项研究中，详细分析了指纹图谱与抗氧化活性之间的蒲公英谱效关系，同时建立了一种 UHPLC 定量方法，用于同时测定蒲公英中的活性成分。通过建立不同地区的蒲公英指纹图谱，鉴定出 24 个共有峰。然后采用经典的 FRAP 法和 ABTS 法检测其抗氧化活性。采用偏最小二乘回归分析、双变量相关分析和灰色关联度法进行谱效关系分析。最终，通过谱效关系分析发现了具有抗氧化活性的成分，可作为蒲公英的候选质量标志物。定量测定了 6 种化合物，包括咖啡酸、绿原酸、咖啡酸、菊苣酸、异绿原酸 A 和异绿原酸 C。所建立的 UHPLC 分析方法准确、精密、可靠。该研究阐明了蒲公英的抗氧化物质基础，为蒲公英的质量控制提供了科学依据。
