云南鸡油菌多糖的结构表征及体外降血糖活性研究

Structural characterization, and in vitro hypoglycemic activity of a polysaccharide from the mushroom Cantharellus yunnanensis.

机构信息

Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development on Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China.

Gongyi Public Traditional Chinese Medicine Hospital, Henan Province, Zhengzhou 451200, China.

出版信息

Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127200. doi: 10.1016/j.ijbiomac.2023.127200. Epub 2023 Oct 2.

DOI:10.1016/j.ijbiomac.2023.127200

PMID:37793536

Abstract

A polysaccharide CY-2 from C. yunnanensis was obtained through a process of consecutive water extraction, alcohol precipitation, and DEAE-52 fast-flow chromatography. CY-2, with an average molecular weight of 2.69 × 10 Da mainly consisted of glucose and mannose with a molar ratio of 33.5: 56.9. Infrared spectrum (IR), methylation analysis, and nuclear magnetic resonance (NMR) results revealed that CY-2 may have a backbone consisting of →6)-α-D-Manp-(1 → 3)-β-D-Glcp-(1→, and branch chain β-D-Glcp-(1→. Meanwhile, CY-2 had a higher inhibition rate on α-glucosidase activity compared with other fractions (CY-0, CY-1, and CY-4) and was a mixed competitive inhibitor. In addition, CY-2 at the concentration of 10 μg/mL presented a superior power to improve glucose consumption and metabolism in HepG2 cells compared with metformin. Overall, these findings highlight the potential value of CY-2 as a hypoglycemic agent.

摘要

从云南虫草中提取到一种多糖 CY-2，该多糖通过连续水提、醇沉和 DEAE-52 快速流色谱法得到。CY-2 的平均分子量为 2.69×10 Da，主要由葡萄糖和甘露糖组成，摩尔比为 33.5:56.9。红外光谱（IR）、甲基化分析和核磁共振（NMR）结果表明，CY-2 可能具有由→6）-α-D-Manp-（1→3）-β-D-Glcp-（1→和支链β-D-Glcp-（1→组成的主链。同时，与其他馏分（CY-0、CY-1 和 CY-4）相比，CY-2 对α-葡萄糖苷酶活性的抑制率更高，是一种混合竞争抑制剂。此外，与二甲双胍相比，浓度为 10μg/mL 的 CY-2 能够更好地促进 HepG2 细胞葡萄糖的消耗和代谢。总的来说，这些发现表明 CY-2 具有作为降血糖剂的潜在价值。