Kikut Justyna, Drozd Arleta, Mokrzycka Małgorzata, Grzybowska-Chlebowczyk Urszula, Ziętek Maciej, Szczuko Małgorzata
Department of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 71-460 Szczecin, Poland.
Department of Pediatrics, Hemato-Oncology and Pediatric Gastroenterology, Independent Public Clinical Hospital No.1, Pomeranian Medical University in Szczecin, 71-252 Szczecin, Poland.
J Clin Med. 2022 Apr 24;11(9):2388. doi: 10.3390/jcm11092388.
Recently, an increase in the incidence of inflammatory bowel disease (IBD) has been observed among children and adolescents. Although the pathogenesis of IBD is not fully elucidated currently, actual research focuses on the occurrence of imbalance between pro- and anti-inflammatory molecules and future identification of the role of cytokines in IBD therapy. The purpose of this study was to compare the concentrations of eicosapentaenoic and docosahexaenoic acid derivatives during both phases of Crohn's disease (CD) and ulcerative colitis (UC). The study included 64 adolescent patients with CD ( = 34) and UC ( = 30) aged 13.76 ± 2.69 and 14.15 ± 3.31, respectively. Biochemical analysis was performed on a liquid chromatography apparatus. A statistically significant lower concentration of resolvin E1 (RvE1) was observed in the CD group relative to UC. In the active phase of CD, a statistically significantly higher concentration of protectin DX (PDX) was observed relative to remission CD. Comparing the active phase of both diseases, a statistically significantly higher concentration of resolvin E1 (RvE1) was observed in UC relative to CD. Comparing the remission phase of both diseases showed statistically significantly higher PDX levels in CD relative to UC. Our study adds to the knowledge on the involvement of anti-inflammatory lipid mediators in both IBD entities. In conclusion, it seems that the marker differentiating both disease entities in the active phase may be RvE1, while in the remission phase, PDX. In CD remission, the greatest involvement was observed towards PDX, whereas in UC, MaR1, RvE1 and 18RS-HEPE seem to be the most involved in remission.
最近，在儿童和青少年中观察到炎症性肠病（IBD）的发病率有所上升。尽管目前IBD的发病机制尚未完全阐明，但实际研究集中在促炎和抗炎分子之间失衡的发生以及未来细胞因子在IBD治疗中的作用鉴定。本研究的目的是比较克罗恩病（CD）和溃疡性结肠炎（UC）两个阶段中二十碳五烯酸和二十二碳六烯酸衍生物的浓度。该研究纳入了64例青少年患者，其中CD患者34例，UC患者30例，年龄分别为13.76±2.69岁和14.15±3.31岁。在液相色谱仪上进行生化分析。相对于UC，CD组中观察到消退素E1（RvE1）的浓度在统计学上显著降低。在CD的活动期，相对于缓解期的CD，观察到保护素DX（PDX）的浓度在统计学上显著更高。比较两种疾病的活动期，相对于CD，UC中观察到消退素E1（RvE1）的浓度在统计学上显著更高。比较两种疾病的缓解期，相对于UC，CD中PDX水平在统计学上显著更高。我们的研究增加了关于抗炎脂质介质在两种IBD实体中作用的知识。总之，似乎在活动期区分两种疾病实体的标志物可能是RvE1，而在缓解期是PDX。在CD缓解期，观察到PDX的参与度最高，而在UC中，MaR1、RvE1和18RS-HEPE似乎在缓解期参与度最高。
