Center for Molecular Biology, College of Medicine and Pharmacy, Duy Tan University, Danang, 55000, Vietnam.
Department of Medicine, College of Medicine and Pharmacy, Duy Tan University, Danang, 55000, Vietnam.
World J Microbiol Biotechnol. 2022 May 16;38(7):112. doi: 10.1007/s11274-022-03294-5.
Eriodictyol (ED) is a flavonoid in the flavanones subclass. It is abundantly present in a wide range of medicinal plants, citrus fruits, and vegetables. In addition, ED owns numerous importantly medicinal bioactivities such as inhibition of proliferation, metastasis and induction of apoptosis in glioma cells or inhibition of glioblastoma migration, and invasion. This study described the heterologous production of ED by E. coli based co-culture engineering system from the simple carbon substrate D-glucose. Two E. coli strains were engineered and functioned as constitutive components of biological system. Specifically, the first strain (upstream module) contained genes for synthesis of p-coumaric acid (pCA) from D-glucose. And, the second strain (downstream module) consisted of genes for the synthesis of ED from pCA. The highest yield in ED production was achieved 51.5 ± 0.4 mg/L using stepwise optimal culture conditions, while monoculture was achieved 21.3 ± 0.2 mg/L only. In conclusion, co-culture was the most efficient alternative approach for the synthesis of ED and other natural products.
圣草次苷（ED）是黄酮类化合物中黄烷酮类的一种。它广泛存在于多种药用植物、柑橘类水果和蔬菜中。此外，ED 具有许多重要的药用生物活性，如抑制神经胶质瘤细胞的增殖、转移和诱导细胞凋亡，以及抑制神经胶质瘤的迁移和侵袭。本研究描述了基于大肠杆菌共培养工程系统，从简单碳源 D-葡萄糖异源生产 ED。对两株大肠杆菌进行了工程改造，作为生物系统的组成部分。具体来说，第一株菌（上游模块）含有从 D-葡萄糖合成对香豆酸（pCA）的基因。第二株菌（下游模块）则包含从 pCA 合成 ED 的基因。通过逐步优化培养条件，ED 的最高产量达到 51.5±0.4mg/L，而单培养仅达到 21.3±0.2mg/L。总之，共培养是合成 ED 和其他天然产物最有效的替代方法。
