Tavakoli Forough, Yavarian Jila, Shafiei Jandaghi Nazanin Zahra, Sadeghi Kaveh, Ghavami Nastaran, Salimi Vahid, Mokhtari-Azad Talat
Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
Future Virol. 2022 Apr. doi: 10.2217/fvl-2021-0014. Epub 2022 May 9.
To evaluate SARS-CoV-2 genome detection using pooled samples by RT-qPCR assay, compared to individual samples. At first all samples were tested individually using two commercial methods targeting , and genes. Then, four experimental groups of samples were pooled and evaluated using the same detection methods. Compared to the individual sample testing, the sample pooling conserved the sensitivity of the detection in all groups of pooled samples when the Ct value in single test was lower than 33. Specimen pooling may fail to detect positive samples with high Ct values. However, in scarcity of reagents or in population surveys, it could be considered as an alternative method in low prevalence settings.
通过逆转录定量聚合酶链反应（RT-qPCR）检测法，使用混合样本评估新型冠状病毒2（SARS-CoV-2）基因组，与单个样本检测进行比较。首先，使用两种针对不同基因的商业方法对所有样本进行单独检测。然后，将样本分为四个实验组进行混合，并使用相同的检测方法进行评估。与单个样本检测相比，当单次检测的Ct值低于33时，样本混合检测在所有混合样本组中均保持了检测的灵敏度。样本混合检测可能无法检测出Ct值较高的阳性样本。然而，在试剂短缺或进行人群调查时，在低流行率环境中可将其视为一种替代方法。
