Department of Otolaryngology, The Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Jiangsu, China.
Appl Immunohistochem Mol Morphol. 2022 Jul 1;30(6):469-475. doi: 10.1097/PAI.0000000000001034. Epub 2022 May 17.
To investigate the diagnostic value of casein kinase 1α (CK1α) and phosphatase and tensin homolog (PTEN) in sinonasal inverted papilloma (SNIP), 42 control subjects and 56 SNIP patients were recruited in this study. Demographic and clinical characteristics, computerized tomography scans and endoscopic examinations were analyzed according to the Krouse staging system. Real-time quantitative-polymerase chain reaction and Western blotting were performed to detect CK1α and PTEN expression levels in different subgroups. Receiver operating characteristic and correlation analyses were conducted to assess their clinical significance in SNIP diagnosis. The expression levels of CK1α and PTEN were decreased in SNIP patients. Interestingly, the declined mRNA levels were consistent with the elevated Krouse staging and closely associated with the pathophysiological characteristics. Their expression levels also negatively correlated with neutrophil counts and positively correlated with lymphocyte counts in the blood of SNIP patients. This study suggests that CK1α and PTEN might be useful biomarkers for the occurrence and recurrence diagnosis of SNIP.
为了研究酪蛋白激酶 1α（CK1α）和磷酸酶张力蛋白同源物（PTEN）在鼻腔鼻窦内翻性乳头状瘤（SNIP）中的诊断价值，本研究招募了 42 名对照者和 56 名 SNIP 患者。根据 Krouse 分期系统对患者的人口统计学和临床特征、计算机断层扫描和内镜检查结果进行了分析。采用实时定量聚合酶链反应和 Western blot 检测了不同亚组中 CK1α和 PTEN 的表达水平。通过接受者操作特征和相关性分析评估了它们在 SNIP 诊断中的临床意义。SNIP 患者的 CK1α和 PTEN 表达水平降低。有趣的是，mRNA 水平的下降与升高的 Krouse 分期一致，并与病理生理特征密切相关。它们的表达水平也与 SNIP 患者血液中的中性粒细胞计数呈负相关，与淋巴细胞计数呈正相关。本研究表明，CK1α 和 PTEN 可能是 SNIP 发生和复发诊断的有用生物标志物。
