Controlled Release of TGF-β3 for Effective Local Endogenous Repair in IDD Using Rat Model.

INTRODUCTION

Intervertebral disc (IVD) degeneration (IDD) is one of the most widespread musculoskeletal diseases worldwide and remains an intractable clinical challenge. Currently, regenerative strategies based on biomaterials and biological factors to facilitate IVD repair have been widely explored. However, the harsh microenvironment, such as increased ROS and acidity, of the degenerative region impedes the efficiency of IVD repair. Here, an intelligent biodegradable nanoplatform using hollow manganese dioxide (H-MnO) was developed to modulate the degenerative microenvironment and release transforming growth factor beta-3 (TGF-β3), which may achieve good long-term therapeutic effects on needle puncture-induced IDD.

METHODS

Surface morphology and elemental analysis of the MnO nanoparticles (NPs) were performed by transmission electron microscopy and an energy-dispersive X-ray spectroscopy detector system, respectively. The biological effects of MnO loaded with TGF-β3 (TGF-β3/MnO) on nucleus pulposus cells (NPCs) were assessed via cytoskeleton staining, EdU staining, qPCR and immunofluorescence. The efficacy of TGF-β3/MnO on needle puncture-induced IDD was further examined using MRI and histopathological and immunohistochemical staining.

RESULTS

The MnO NPs had a spherical morphology and hollow structure that dissociated in the setting of a low pH and HO to release loaded TGF-β3 molecules. In the oxidative stress environment, TGF-β3/MnO was superior to TGF-β3 and MnO NPs in the suppression of HO-induced matrix degradation, ROS, and apoptosis in NPCs. When injected into the IVDs of a rat IDD model, TGF-β3/MnO was able to prevent the degeneration and promote self-regeneration.

CONCLUSION

Use of an MnO nanoplatform for biological factors release to regulate the IDD microenvironment and promote endogenous repair may be an effective approach for treating IDD.