Laboratory of Microbial Biotechnology, Enzymatic and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sidi Mansour Road Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
Department of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas (CIB-CSIC), C/ Ramiro de Maeztu 9, 28040, Madrid, Spain.
Microb Cell Fact. 2022 May 21;21(1):91. doi: 10.1186/s12934-022-01818-0.
Omics approaches are widely applied in the field of biology for the discovery of potential CAZymes including whole genome sequencing. The aim of this study was to identify protein encoding genes including CAZymes in order to understand glycans-degrading machinery in the thermophilic Caldicoprobacter algeriensis TH7C1 strain.
Caldicoprobacter algeriensis TH7C1 is a thermophilic anaerobic bacterium belonging to the Firmicutes phylum, which grows between the temperatures of 55 °C and 75 °C. Next generation sequencing using Illumina technology was performed on the C. algeriensis strain resulting in 45 contigs with an average GC content of 44.9% and a total length of 2,535,023 bp. Genome annotation reveals 2425 protein-coding genes with 97 ORFs coding CAZymes. Many glycoside hydrolases, carbohydrate esterases and glycosyltransferases genes were found linked to genes encoding oligosaccharide transporters and transcriptional regulators; suggesting that CAZyme encoding genes are organized in clusters involved in polysaccharides degradation and transport. In depth analysis of CAZomes content in C. algeriensis genome unveiled 33 CAZyme gene clusters uncovering new enzyme combinations targeting specific substrates.
This study is the first targeting CAZymes repertoire of C. algeriensis, it provides insight to the high potential of identified enzymes for plant biomass degradation and their biotechnological applications.
组学方法广泛应用于生物学领域，用于发现潜在的 CAZymes，包括全基因组测序。本研究的目的是鉴定编码蛋白质的基因，包括 CAZymes，以了解嗜热的 Algeriensis 热球菌 TH7C1 菌株中的聚糖降解机制。
Algeriensis 热球菌 TH7C1 是一种属于Firmicutes 门的嗜热厌氧细菌，在 55°C 和 75°C 之间生长。对 Algeriensis 菌株进行基于 Illumina 技术的下一代测序，得到 45 个 contigs，平均 GC 含量为 44.9%，总长度为 2535023bp。基因组注释显示有 2425 个编码蛋白的基因，其中 97 个 ORF 编码 CAZymes。发现许多糖苷水解酶、碳水化合物酯酶和糖基转移酶基因与编码寡糖转运蛋白和转录调节因子的基因相连；这表明 CAZyme 编码基因组织在涉及多糖降解和运输的簇中。对 Algeriensis 基因组中 CAZomes 内容的深入分析揭示了 33 个 CAZyme 基因簇，揭示了针对特定底物的新酶组合。
本研究首次针对 Algeriensis 的 CAZymes 库进行了研究，为鉴定出的酶在植物生物质降解及其生物技术应用中的高潜力提供了深入了解。
