Department of Life Science, Dongguk University Seoul, Goyang, 10326, South Korea.
Antonie Van Leeuwenhoek. 2022 Jul;115(7):943-952. doi: 10.1007/s10482-022-01748-2. Epub 2022 May 23.
A Gram-stain-positive, aerobic, motile and rod-shaped bacterium, designated RG28, was isolated from the roots of rice plant collected from paddy fields in Ilsan, South Korea. Cells of the strain were oxidase-negative but catalase-positive. Strain RG28 was found to grow at 10-50 °C (optimum, 25-30 °C), pH 5.0-10.0 (optimum, pH 7.0) and in 1.0-5.0% (w/v) NaCl (optimum, 0%). The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinones were MK-7 and MK-6. The predominant cellular fatty acids were C, iso-C and anteiso-C The major polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, four unidentified aminophosphoglycolipids, four unidentified aminophospholipids, two unidentified glycolipids, one unidentified aminoglycolipid and four unidentified lipids. The genomic DNA G + C content was 33.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Gottfriedia acidiceleris CBD 119 (98.6%), Gottfriedia solisilvae LMG 18422 (98.5%) and Gottfriedia luciferensis LMG 18422 (98.4%). The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) values between strain RG28 and type strains of Gottfriedia species were lower than the cut-offs (≥ 95-96% for ANI and ≥ 70% for is DDH) required to define a bacterial species. Meanwhile, the strain has the ability to produce indole-acetic acid (40.5 µg/mL). Phylogenetic, physiological and chemotaxonomic data suggested that strain RG28 represented a novel species of the genus Gottfriedia, for which the name Gottfriedia endophyticus sp. nov. is proposed, with the type strain RG28 (= KCTC 43327 = TBRC 15151).
一株革兰氏阳性、需氧、能运动和杆状的细菌，被命名为 RG28，从韩国 Ilsan 的稻田中采集的水稻根部分离得到。该菌株的细胞氧化酶阴性，但过氧化氢酶阳性。发现菌株 RG28 在 10-50°C（最佳温度为 25-30°C）、pH5.0-10.0（最佳 pH 值为 7.0）和 1.0-5.0%（w/v）NaCl（最佳为 0%）的条件下生长。细胞壁肽聚糖含有meso-二氨基庚二酸，主要的menaquinones 为 MK-7 和 MK-6。主要的细胞脂肪酸为 C、iso-C 和 anteiso-C。主要的极性脂包括磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、四种未鉴定的氨基糖脂、四种未鉴定的氨基磷脂、两种未鉴定的糖脂、一种未鉴定的氨基糖脂和四种未鉴定的脂类。基因组 DNA G+C 含量为 33.5 mol%。基于 16S rRNA 基因序列的系统发育分析表明，该菌株与 Gottfriedia acidiceleris CBD 119（98.6%）、Gottfriedia solisilvae LMG 18422（98.5%）和 Gottfriedia luciferensis LMG 18422（98.4%）密切相关。与 Gottfriedia 属的模式菌株相比，菌株 RG28 的平均核苷酸同一性（ANI）和体外 DNA-DNA 杂交（isDDH）值均低于定义细菌种所需的截止值（ANI≥95-96%，isDDH≥70%）。同时，该菌株具有产生吲哚乙酸（40.5µg/mL）的能力。系统发育、生理和化学分类学数据表明，菌株 RG28 代表 Gottfriedia 属的一个新种，建议将其命名为 Gottfriedia endophyticus sp. nov.，其模式菌株为 RG28（=KCTC 43327=TBRC 15151）。
