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热带类伯克霍尔德氏菌Ppe8菌株的基因组草图序列,一种甘蔗内生固氮细菌。

Draft genome sequence of Paraburkholderia tropica Ppe8 strain, a sugarcane endophytic diazotrophic bacterium.

作者信息

Silva Paula Renata Alves da, Simões-Araújo Jean Luiz, Vidal Márcia Soares, Cruz Leonardo Magalhães, Souza Emanuel Maltempi de, Baldani José Ivo

机构信息

Embrapa Agrobiologia, Seropédica, RJ, Brazil.

Universidade Federal do Paraná - UFPR, Curitiba, PR, Brazil.

出版信息

Braz J Microbiol. 2018 Apr-Jun;49(2):210-211. doi: 10.1016/j.bjm.2017.07.005. Epub 2017 Oct 18.

Abstract

Paraburkholderia tropica (syn Burkholderia tropica) are nitrogen-fixing bacteria commonly found in sugarcane. The Paraburkholderia tropica strain Ppe8 is part of the sugarcane inoculant consortium that has a beneficial effect on yield. Here, we report a draft genome sequence of this strain elucidating the mechanisms involved in its interaction mainly with Poaceae. A genome size of approximately 8.75Mb containing 7844 protein coding genes distributed in 526 subsystems was de novo assembled with ABySS and annotated by RAST. Genes related to the nitrogen fixation process, the secretion systems (I, II, III, IV, and VI), and related to a variety of metabolic traits, such as metabolism of carbohydrates, amino acids, vitamins, and proteins, were detected, suggesting a broad metabolic capacity and possible adaptation to plant association.

摘要

热带类伯克霍尔德氏菌(同义词:热带伯克霍尔德氏菌)是常见于甘蔗中的固氮细菌。热带类伯克霍尔德氏菌菌株Ppe8是甘蔗接种剂联合体的一部分,对产量有有益影响。在此,我们报告该菌株的基因组草图序列,阐明其主要与禾本科植物相互作用所涉及的机制。使用ABySS软件对大小约为8.75Mb的基因组进行了从头组装,该基因组包含分布在526个亚系统中的7844个蛋白质编码基因,并通过RAST进行注释。检测到与固氮过程、分泌系统(I、II、III、IV和VI)以及与多种代谢特征相关的基因,如碳水化合物、氨基酸、维生素和蛋白质的代谢,这表明该菌株具有广泛的代谢能力以及对与植物共生的可能适应性。

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