Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa.
Human Sciences Research Council, Pretoria 0083, South Africa.
Molecules. 2023 Jun 8;28(12):4654. doi: 10.3390/molecules28124654.
Engineering research has been expanded by the advent of material fusion, which has led to the development of composites that are more reliable and cost-effective. This investigation aims to utilise this concept to promote a circular economy by maximizing the adsorption of silver nanoparticles and silver nitrate onto recycled chicken eggshell membranes, resulting in optimized antimicrobial silver/eggshell membrane composites. The pH, time, concentration, and adsorption temperatures were optimized. It was confirmed that these composites were excellent candidates for use in antimicrobial applications. The silver nanoparticles were produced through chemical synthesis using sodium borohydride as a reducing agent and through adsorption/surface reduction of silver nitrate on eggshell membranes. The composites were thoroughly characterized by various techniques, including spectrophotometry, atomic absorption spectrometry, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy, as well as agar well diffusion and MTT assay. The results indicate that silver/eggshell membrane composites with excellent antimicrobial properties were produced using both silver nanoparticles and silver nitrate at a pH of 6, 25 °C, and after 48 h of agitation. These materials exhibited remarkable antimicrobial activity against and , resulting in 27.77% and 15.34% cell death, respectively.
工程研究随着材料融合的出现而得到扩展，这导致了更可靠和更具成本效益的复合材料的发展。本研究旨在利用这一概念，通过最大限度地吸附银纳米粒子和硝酸银到回收的鸡蛋壳膜上来促进循环经济，从而得到优化的抗菌银/蛋壳膜复合材料。优化了 pH 值、时间、浓度和吸附温度。证实这些复合材料是抗菌应用的优秀候选材料。通过使用硼氢化钠作为还原剂的化学合成和通过硝酸银在蛋壳膜上的吸附/表面还原来制备银纳米粒子。通过各种技术，包括分光光度法、原子吸收光谱法、扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱和 X 射线光电子能谱以及琼脂孔扩散和 MTT 测定法，对复合材料进行了彻底的表征。结果表明，在 pH 值为 6、25°C 并搅拌 48 小时的条件下，使用银纳米粒子和硝酸银都可以制备出具有优异抗菌性能的银/蛋壳膜复合材料。这些材料对 和 表现出显著的抗菌活性，导致细胞死亡率分别为 27.77%和 15.34%。
