National Traditional Chinese Medicine Clinical Research Base and Cardiovascular Medicine Department of the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 646000 Sichuan, China.
College of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, 646000 Sichuan, China.
Comput Math Methods Med. 2022 May 16;2022:3900828. doi: 10.1155/2022/3900828. eCollection 2022.
Stable angina pectoris (SAP) is one of the main types of coronary heart disease (CHD). To improve treatment outcomes, more effective biomarkers are needed. Currently, studies on the metabolic characteristics of SAP are lacking. Here, we explored the serum metabolomic profile of SAP and identified potential biomarkers and related pathways to assist the clinical diagnosis and treatment of SAP.
Thirty patients with SAP patients and 30 healthy controls (CON) without stenosis were selected for this study. All patients underwent coronary angiography. The metabolites of the two groups' serum samples were investigated using UHPLC-QE-MS. Changes in serum metabolic profile were evaluated using multivariate statistical analysis and pathway analysis.
OPLS-DA analysis identified significant differences in the serum metabolic profiles between patients with SAP and CON. Twenty-five differential metabolites were identified between patients from SAP and CON groups, including choline, creatine, L-arginine, beta-guanidinopropionic acid, isopalmitic acid, xanthine, LysoPC (18 : 1), and LysoPC (20 : 3). Pathway analysis found that these differential metabolites were involved in energy metabolism, oxidative stress, purine metabolism, and other metabolic pathways.
By comparing the serum metabolic profiles of SAP patients with a control group, we identified 25 potential biomarkers that could improve the clinical diagnosis and treatment of SAP.
稳定型心绞痛（SAP）是冠心病（CHD）的主要类型之一。为了改善治疗效果，需要更有效的生物标志物。目前，SAP 的代谢特征研究还比较缺乏。在这里，我们探讨了 SAP 的血清代谢组学特征，并确定了潜在的生物标志物和相关途径，以协助 SAP 的临床诊断和治疗。
本研究选取了 30 例 SAP 患者和 30 例无狭窄的健康对照者（CON）。所有患者均接受了冠状动脉造影。采用 UHPLC-QE-MS 对两组血清样本的代谢物进行了研究。采用多变量统计分析和途径分析评估血清代谢谱的变化。
OPLS-DA 分析发现 SAP 患者和 CON 之间的血清代谢谱存在显著差异。在 SAP 组和 CON 组之间鉴定出 25 种差异代谢物，包括胆碱、肌酸、L-精氨酸、β-胍基丙酸、异棕榈酸、黄嘌呤、溶血磷脂酰胆碱（18：1）和溶血磷脂酰胆碱（20：3）。途径分析发现这些差异代谢物参与了能量代谢、氧化应激、嘌呤代谢和其他代谢途径。
通过比较 SAP 患者和对照组的血清代谢谱，我们确定了 25 种潜在的生物标志物，这些标志物可能改善 SAP 的临床诊断和治疗。
