Jia Huanyu, Muhae-Ud-Din Ghulam, Zhang Han, Zong Qianqian, Zhao Sifeng, Guo Qingyuan, Chen Wanquan, Gao Li
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Key Laboratory at Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization, Shihezi University, Xinjiang, China.
Front Microbiol. 2022 May 9;13:853176. doi: 10.3389/fmicb.2022.853176. eCollection 2022.
Rhizosphere soil microorganisms have great agricultural importance. To explore the relationship between rhizosphere microorganisms and the disease incidence, and to optimize the concentration of difenoconazole fungicide for the control of wheat dwarf bunt, caused by Kühn, the rhizosphere microorganisms were characterized based on sequencing methods. We found that the disease incidence correlated with the relative abundance of some microbial communities, such as Acidobacteria, Nocardioides, Roseiflexaceae, Pyrinomonadaceae, and Gemmatimonadaceae. Actinobacteria showed significant differences in the infected soils when compared to the control soils, and the relative abundance of Acidobacteria, Pyrinomonadaceae, Gemmatimonadaceae, and Saccharimonadales populations was distinctly higher in the -inoculated group than in the control group. The members of Dehalococcoidia, Nitrosomonadaceae, and Thermomicrobiales were found only in -inoculated soils, and these taxa may have potential effects against the pathogen and contribute to disease control of wheat dwarf bunt. In addition, for -infected plants, the soil treated with difenoconazole showed a high relative abundance of Proteobacteria, Actinobacteria, Ascomycota, Basidiomycota, Mortierellomycota, and Olpidiomycota based on the heatmap analysis and ANOVA. Our findings suggest that the optimized concentration of fungicide (5% recommended difenoconazole) exhibits better control efficiency and constant diversity in the rhizosphere soil.
根际土壤微生物具有重大的农业意义。为了探究根际微生物与发病率之间的关系，并优化苯醚甲环唑杀菌剂对由 Kühn 引起的小麦矮腥黑穗病的防治浓度，基于测序方法对根际微生物进行了表征。我们发现发病率与一些微生物群落的相对丰度相关，如酸杆菌门、诺卡氏菌属、玫瑰弯菌科、梨形单胞菌科和芽单胞菌门。与对照土壤相比，放线菌在受感染土壤中表现出显著差异，接种组中酸杆菌门、梨形单胞菌科、芽单胞菌门和糖单胞菌目的相对丰度明显高于对照组。仅在接种土壤中发现了脱卤球菌纲、亚硝化单胞菌科和嗜热微生物目的成员，这些分类群可能对病原体具有潜在作用，并有助于小麦矮腥黑穗病的病害防治。此外，对于受感染的植物，基于热图分析和方差分析，用苯醚甲环唑处理的土壤中变形菌门、放线菌门、子囊菌门、担子菌门、被孢霉门和油壶菌门的相对丰度较高。我们的研究结果表明，优化后的杀菌剂浓度（推荐浓度的 5%苯醚甲环唑）在根际土壤中表现出更好的防治效果和稳定的多样性。
