State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Key Laboratory at Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization, Shihezi University, Xinjiang, 832003, China.
Microb Ecol. 2021 Oct;82(3):736-745. doi: 10.1007/s00248-021-01696-w. Epub 2021 Feb 1.
Tilletia foetida (syn. T. laevis) leads to wheat common bunt, a worldwide disease that can lead to 80% yield loss and even total loss of production, together with degrading the quality of grains and flour by producing a rotten fish smell. To explore the potential microbial community that may contribute to the control of soil- and seed-borne pathogens, in this study, we analyzed the effects of the plant pathogenic fungus T. foetida on rhizosphere soil microorganisms in wheat seeds coated with different concentrations of a fungicide (Jianzhuang) used to control the disease. To analyze the bacterial and fungal abundance in T. foetida-infected and mock-infected plants, the microorganisms were sequenced using high-throughput HiSeq 2500 gene sequencing. The results showed that bacterial communities, including Verrucomicrobia, Patescibacteria, Armatimonadetes, Nitrospirae, Fibrobacteres, Chlamydiae, and Hydrogenedentes, and fungal communities, including Basidiomycota and Ciliophora, were more prevalent in the mock group than in the T. foetida-infected group, which may contribute to the control of wheat common bunt. Moreover, cluster and PCoA analysis revealed that replicates of the same samples were clustered together, and these results were also found in the distance index within-group analysis for bacterial and fungal communities in the T. foetida-infected and mock groups.
腥黑粉菌（syn. 光滑腥黑粉菌）可导致小麦散黑穗病，这是一种世界性病害，可导致 80%的产量损失，甚至完全减产，同时通过产生腐鱼臭味来降低谷物和面粉的质量。为了探索可能有助于控制土壤和种子传播病原体的潜在微生物群落，在本研究中，我们分析了植物病原真菌腥黑粉菌对用杀菌剂（健壮）包衣的小麦种子根际土壤微生物的影响，该杀菌剂用于控制该病害。为了分析感染和未感染腥黑粉菌的植物中的细菌和真菌丰度，使用高通量 HiSeq 2500 基因测序对微生物进行了测序。结果表明，细菌群落，包括疣微菌门、Patescibacteria 门、变形菌门、硝化螺旋菌门、纤维杆菌门、衣原体门和氢噬菌门，以及真菌群落，包括担子菌门和纤毛门，在模拟组中比在腥黑粉菌感染组中更为普遍，这可能有助于控制小麦散黑穗病。此外，聚类和 PCoA 分析表明，相同样本的重复样本聚集在一起，在腥黑粉菌感染组和模拟组的细菌和真菌群落的组内距离指数分析中也发现了这一结果。
