Department of Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, Hassan II University, Casablanca, Morocco.
Department of Biological Sciences, Faculty of Medicine, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco.
Microrna. 2022;11(3):190-205. doi: 10.2174/2211536611666220523103316.
MicroRNAs (miRNAs) are small noncoding RNA molecules involved in the post-transcriptional regulation of genes. Deregulated expression of miRNAs is involved in different pathogenic mechanisms, particularly colorectal cancer (CRC) carcinogenesis. Due to their stability and accessibility, circulating miRNAs represent a new family of biomarkers with great potential. Therefore, certain miRNAs can be used as diagnostic biomarkers in CRC.
This systematic analysis aimed to explore the individual efficacy of the most investigated blood-based miRNAs for CRC diagnosis, namely miR-21, miR-29a and miR-92a.
Articles were retrieved from databases such as PubMed and Google Scholar, and studies designed to evaluate the diagnostic value of microRNAs in CRC were then selected. We subsequently explored the diagnostic accuracy of each miRNA using parameters such as (SE, SPE, PLR, NLR). The meta-analysis was performed using the Review Manager (Revman) 5.4 software and the Meta Disc software.
Our results suggested that serum miR-21 levels showed great potential as a diagnostic molecular marker. The overall pooled results for sensitivity, specificity, area under the curve (AUC), PLR, and NLR were 78%, 91%, 0.9519, 8.12 and 0.17, respectively.
miRNAs have become increasingly important in the diagnosis of CRC. Based on these findings, circulating miR-21 levels may have a potential value for early detection and might be used as a novel diagnostic biomarker for CRC.
微小 RNA（miRNA）是参与基因转录后调控的小非编码 RNA 分子。miRNA 的表达失调与不同的发病机制有关，特别是结直肠癌（CRC）的发生。由于其稳定性和可及性，循环 miRNA 代表了具有巨大潜力的新型生物标志物家族。因此，某些 miRNA 可以作为 CRC 的诊断生物标志物。
本系统分析旨在探讨研究最多的血液 miRNA 作为 CRC 诊断的个体疗效，即 miR-21、miR-29a 和 miR-92a。
从 PubMed 和 Google Scholar 等数据库中检索文章，并选择旨在评估 microRNA 在 CRC 中的诊断价值的研究。然后，我们使用（SE、SPE、PLR、NLR）等参数探索每种 miRNA 的诊断准确性。使用 Review Manager（Revman）5.4 软件和 Meta Disc 软件进行荟萃分析。
我们的结果表明，血清 miR-21 水平具有作为诊断分子标志物的巨大潜力。敏感性、特异性、曲线下面积（AUC）、PLR 和 NLR 的汇总结果分别为 78%、91%、0.9519、8.12 和 0.17。
miRNA 在 CRC 的诊断中变得越来越重要。基于这些发现，循环 miR-21 水平可能具有早期检测的潜在价值，并可能用作 CRC 的新型诊断生物标志物。
