Ho Yolanda, Gyurko Robert, Uzel Naciye Guzin, Steffensen Bjorn, Xenoudi Pinelopi, Loo Cheen, Zoukhri Driss
Department of Periodontology, Tufts University School of Dental Medicine, Boston, MA, USA.
College of Dental Medicine, California Northstate University, Sacramento, CA, USA.
Int J Dent. 2022 May 17;2022:9439096. doi: 10.1155/2022/9439096. eCollection 2022.
The aims of this study were to investigate the antibacterial and cytotoxic effects of silver diamine fluoride (SDF) on periodontal pathogens and human skin constructs, respectively.
SDF has been proven to have bactericidal effects on cariogenic bacteria. No studies to date evaluated the bactericidal effects of SDF on periodontal pathogens nor its effect on epithelium and fibroblasts.
and were cultured in monospecies biofilms, exposed to increasing concentrations of SDF and inoculated on agar plates to assess viability. Human gingival fibroblasts in 2D cultures were exposed to 1 L of 0.394% of SDF and viewed using real-time imaging. Finally, SDF was applied to human, 3D tissue scaffolds of fibroblasts and keratinocytes, and termed human skin equivalents (HSE). A clinical dose of 38% SDF was applied, and HSE were cultured for 12 hours, 1, 3, 5, and 10 days. The tissue was observed clinically and histologically with hematoxylin and eosin staining and TUNEL.
and growth was completely inhibited using all dilutions of SDF, whereas was still viable with 0.197% and 0.098% of SDF. Single-layer fibroblasts experienced immediate necrosis upon contact with SDF. Application of SDF to HSE showed maturation of a whitish lesion within 24 hours, followed by pigmented, crusted tissue after 3 days. Histological evaluation of treated tissues showed apoptotic cells in the epithelium and upper half of the connective tissue.
Our data suggest that SDF has bactericidal properties against two periodontal pathogens: and . SDF caused immediate necrosis of monolayer fibroblasts, but does not extend to the full extent of layered fibroblasts in HSE.
本研究旨在分别探究氟化亚锡（SDF）对牙周病原体和人类皮肤构建物的抗菌及细胞毒性作用。
已证实SDF对致龋菌具有杀菌作用。迄今为止，尚无研究评估SDF对牙周病原体的杀菌作用及其对上皮细胞和成纤维细胞的影响。
[具体细菌名称]在单菌种生物膜中培养，暴露于浓度递增的SDF中，然后接种于琼脂平板上以评估活力。二维培养的人牙龈成纤维细胞暴露于1升0.394%的SDF中，并使用实时成像进行观察。最后，将SDF应用于成纤维细胞和角质形成细胞的人类三维组织支架，即人类皮肤等效物（HSE）。应用临床剂量的38% SDF，将HSE培养12小时、1天、3天、5天和10天。通过苏木精和伊红染色及TUNEL对组织进行临床和组织学观察。
所有稀释度的SDF均完全抑制了[具体细菌名称]的生长，而[另一种具体细菌名称]在0.197%和0.098%的SDF作用下仍具有活力。单层成纤维细胞与SDF接触后立即发生坏死。将SDF应用于HSE后，24小时内出现白色病变成熟，3天后出现色素沉着、结痂组织。对处理后组织的组织学评估显示上皮细胞和结缔组织上半部分存在凋亡细胞。
我们的数据表明，SDF对两种牙周病原体[具体细菌名称]具有杀菌特性。SDF导致单层成纤维细胞立即坏死，但并未扩展至HSE中分层成纤维细胞的全部范围。
