Ares Ana M, Tapia Jesús A, González-Porto Amelia V, Higes Mariano, Martín-Hernández Raquel, Bernal José
I. U. CINQUIMA, Analytical Chemistry Group (TESEA), Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain.
Department of Statistics and Operations Research, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain.
Foods. 2022 May 17;11(10):1446. doi: 10.3390/foods11101446.
Bee pollen is currently one of the most commonly consumed food supplements, as it is considered to be a good source of bioactive substances and energy. It contains various health-promoting compounds, such as proteins, amino acids, lipids, as well as glucosinolates. In the present study, the glucosinolate content was determined, by means of ultra-performance liquid chromatography coupled to a quadrupole time-of-flight mass detector, in 72 bee pollen samples from four different apiaries in Guadalajara (Spain), harvested in three different periods. In addition, 11 commercial multifloral samples from different Spanish regions were also analyzed. The aim was to verify the suitability of these compounds as biomarkers of their geographical origin, and to test their potential for distinguishing the harvesting period. By means of a canonical discriminant analysis, it was possible to differentiate the apiary of origin of most of the samples, and these could also be clearly differentiated from the commercial ones, simply as a result of the glucosinolate content. In addition, it was also demonstrated for the first time that bee pollen samples were capable of being differentiated according to the time of harvesting and their glucosinolate content.
蜂花粉是目前最常食用的食品补充剂之一，因为它被认为是生物活性物质和能量的良好来源。它含有各种促进健康的化合物，如蛋白质、氨基酸、脂质以及硫代葡萄糖苷。在本研究中，采用超高效液相色谱与四极杆飞行时间质谱检测器联用的方法，对来自西班牙瓜达拉哈拉四个不同养蜂场的72份蜂花粉样本进行了硫代葡萄糖苷含量测定，这些样本在三个不同时期采集。此外，还分析了来自西班牙不同地区的11份商业多花型样本。目的是验证这些化合物作为其地理来源生物标志物的适用性，并测试它们区分收获期的潜力。通过典型判别分析，可以区分大多数样本的来源养蜂场，而且仅根据硫代葡萄糖苷含量，这些样本也能与商业样本明显区分开来。此外，还首次证明了蜂花粉样本能够根据收获时间及其硫代葡萄糖苷含量进行区分。
