Gonçalves Ana C, Lahlou Radhia Aitfella, Alves Gilberto, Garcia-Viguera Cristina, Moreno Diego A, Silva Luís R
CICS-UBI-Health Sciences Research Centre, Department of Medical Science, University of Beira Interior, 6201-506 Covilhã, Portugal.
Chemistry Department, University of Beira Interior, 6201-001 Covilhã, Portugal.
Foods. 2021 Nov 15;10(11):2804. doi: 10.3390/foods10112804.
The aim of this study was to determine the grain composition and (poly)phenolic profile of pollen from Abrantes (Portugal), as well as its antioxidative and antidiabetic properties, and abilities to protect human erythrocytes against induced hemoglobin oxidation, lipid peroxidation, and hemolysis. The phytochemical profile of the Abrantes' bee pollen revealed twenty phenolic compounds, identified by high-performance liquid chromatography with electrospray ionization mass spectrometry coupled with photodiode array detection. Among them, quercetin derivatives were the most abundant. Concerning the biological potential, the pollen extract showed notable capacity for 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, and superoxide radicals, as well as for inhibition of α-glucosidase action, and protection of human erythrocytes against oxidative damage. Non-cytotoxic effects regarding the NHDF normal cell line, human adenocarcinoma Caco-2, and human liver HepG2 cells were observed. The results obtained contributed to further research on modes of action related to oxidative damage and metabolic health problems, to generate deeper knowledge of potential health-promoting effects to develop novel pharmaceutical drugs, nutraceuticals, and dietary supplements.
本研究的目的是确定来自葡萄牙阿布朗什的花粉的颗粒组成和（多）酚类成分，以及其抗氧化和抗糖尿病特性，以及保护人类红细胞免受诱导的血红蛋白氧化、脂质过氧化和溶血的能力。阿布朗什蜂花粉的植物化学特征显示有20种酚类化合物，通过高效液相色谱-电喷雾电离质谱联用光电二极管阵列检测进行鉴定。其中，槲皮素衍生物最为丰富。关于生物活性，花粉提取物对2,2-二苯基-1-苦基肼自由基、一氧化氮和超氧阴离子自由基具有显著清除能力，对α-葡萄糖苷酶活性有抑制作用，并且能保护人类红细胞免受氧化损伤。对NHDF正常细胞系、人腺癌Caco-2细胞和人肝癌HepG2细胞未观察到细胞毒性作用。所获得的结果有助于进一步研究与氧化损伤和代谢健康问题相关的作用模式，以更深入了解潜在的健康促进作用，从而开发新型药物、营养保健品和膳食补充剂。
