Li Jian, Shao Yanping, Yang Yayong, Xu Chang, Jing Zhuohan, Li Hui, Xie Baogui, Tao Yongxin
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Fungi (Basel). 2022 May 3;8(5):477. doi: 10.3390/jof8050477.
Stipe elongation is an important process in the development of the fruiting body and is associated with the commodity quality of agaric fungi. In this study, was used as a model agaric fungus to reveal the function of the chromatin modifier gene containing the JmjC domain in stipe elongation. First, we identified a JmjC domain family gene () with a 3684 bp length open reading frame (ORF) in . was predicted to have a histone H3K9 demethylation function, and was specifically upregulated during stipe rapid elongation. Further investigation revealed that the silencing of inhibited the mycelial growth, while overexpression of this gene had no effect on the mycelial growth. Comparative analysis revealed that the stipe elongation rate in overexpression strains was significantly increased, while it was largely reduced when was silenced. Taken together, these results suggest that positively regulates the mycelial growth and controls the elongation speed and the length of the stipe. Moreover, cell wall-related enzymes genes, including three exo-β-1,3-glucanases, one β-1,6-glucan synthase, four chitinases, and two expansin proteins, were found to be regulated by . Based on the putative functions of , we propose that this gene enhances the transcription of cell wall-related enzymes genes by demethylating histone H3K9 sites to regulate remodeling of the cell wall in rapid stipe elongation. This study provides new insight into the mechanism of rapid stipe elongation, and it is important to regulate the commodity quality of agaric fungi.
菌柄伸长是子实体发育中的一个重要过程，并且与伞菌的商品品质相关。在本研究中，（此处原文缺失具体菌株名称）被用作模式伞菌，以揭示含有JmjC结构域的染色质修饰基因在菌柄伸长中的功能。首先，我们在（此处原文缺失具体菌株名称）中鉴定出一个长度为3684 bp开放阅读框（ORF）的JmjC结构域家族基因（此处原文缺失具体基因名称）。该基因被预测具有组蛋白H3K9去甲基化功能，并且在菌柄快速伸长期间特异性上调。进一步研究表明，该基因的沉默抑制了菌丝体生长，而该基因的过表达对菌丝体生长没有影响。比较分析表明，该基因过表达菌株的菌柄伸长率显著增加，而当该基因被沉默时，菌柄伸长率大幅降低。综上所述，这些结果表明该基因正向调节菌丝体生长并控制菌柄的伸长速度和长度。此外，发现细胞壁相关酶基因，包括三种外切-β-1,3-葡聚糖酶、一种β-1,6-葡聚糖合酶、四种几丁质酶和两种扩张蛋白，受该基因调控。基于该基因的推定功能，我们提出该基因通过使组蛋白H3K9位点去甲基化来增强细胞壁相关酶基因的转录，从而在菌柄快速伸长过程中调节细胞壁重塑。本研究为菌柄快速伸长的机制提供了新的见解，并且对于调控伞菌的商品品质具有重要意义。
