Seliem Esraa M, Azab Mohamed E, Ismail Randa S, Nafeaa Abeer A, Alotaibi Badriyah S, Negm Walaa A
Department of Physiology, Faculty of Veterinary Medicine, Benha University, Benha 13512, Egypt.
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Life (Basel). 2022 May 6;12(5):693. doi: 10.3390/life12050693.
Obesity is a serious public health issue worldwide. Finding safe and efficacious products to reverse obesity has proven to be a difficult challenge. This study showed the effects of or green coffee bean extract (GCBE) on obesity disorders and the improvement of obesity-induced insulin resistance, dyslipidemia, and inflammation. The active constituents of GCBE were identified via high-performance liquid chromatography. Twenty-four male albino Wistar rats were divided into two groups. The first group (Group I) was fed a control diet, whereas the second group was fed a high-fat diet (HFD) for eight weeks till obesity induction. The second group was equally subdivided into Group II, which received HFD, and Group III, which received HFD + GCBE for another eight weeks. The body and organ weights of the animals were measured, and blood and adipose tissue samples were collected for analysis. The results indicated that the administration of GCBE significantly decreased the body and organ weights. Furthermore, it had an ameliorative effect on serum biochemical parameters. It dramatically reduced total cholesterol, triacylglycerol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, glucose, and insulin levels. In addition, an improvement in homeostasis model assessment-insulin resistance and an enhancement of high-density lipoprotein cholesterol levels were observed compared with the HFD group. In addition, the group treated with GCBE exhibited a marked increase in serum levels of adiponectin (an anti-inflammatory adipokine). In addition, a considerable reduction in adipocyte hypertrophy was found following GCBE treatment. Remarkably, the administration of GCBE resulted in a remarkable decrease in the expression of RBP4 (a pro-inflammatory cytokine), whereas an increase in GLLUT4 expression was observed in the adipose tissue. This improved insulin resistance in GCBE-administered HFD rats compared with other HFD rats. Our study showed that GCBE exhibits anti-obesity activity and may be used as a natural supplement to prevent and treat obesity and its associated disorders.
肥胖是全球范围内一个严重的公共卫生问题。事实证明，找到安全有效的产品来逆转肥胖是一项艰巨的挑战。本研究显示了[具体物质]或绿咖啡豆提取物（GCBE）对肥胖症的影响以及对肥胖诱导的胰岛素抵抗、血脂异常和炎症的改善作用。通过高效液相色谱法鉴定了GCBE的活性成分。将24只雄性白化Wistar大鼠分为两组。第一组（I组）喂食对照饮食，而第二组喂食高脂饮食（HFD）八周直至诱导肥胖。第二组又平均分为接受HFD的II组和接受HFD + GCBE的III组，再持续八周。测量动物的体重和器官重量，并采集血液和脂肪组织样本进行分析。结果表明，给予GCBE显著降低了体重和器官重量。此外，它对血清生化参数有改善作用。它显著降低了总胆固醇、三酰甘油、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇、葡萄糖和胰岛素水平。此外，与HFD组相比，观察到稳态模型评估-胰岛素抵抗有所改善，高密度脂蛋白胆固醇水平有所提高。此外，用GCBE治疗的组血清脂联素（一种抗炎脂肪因子）水平显著升高。此外，GCBE治疗后发现脂肪细胞肥大明显减少。值得注意的是，给予GCBE导致视黄醇结合蛋白4（一种促炎细胞因子）的表达显著降低，而在脂肪组织中观察到葡萄糖转运蛋白4表达增加。与其他HFD大鼠相比，这改善了给予GCBE的HFD大鼠的胰岛素抵抗。我们的研究表明，GCBE具有抗肥胖活性，可作为预防和治疗肥胖及其相关疾病的天然补充剂。
