Teeranachaideekul Veerawat, Parichatikanond Warisara, Junyaprasert Varaporn Buraphacheep, Morakul Boontida
Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Pharmaceuticals (Basel). 2022 Jul 27;15(8):930. doi: 10.3390/ph15080930.
Pumpkin seed oil (PSO)-loaded niosomes were prepared from Tween 20 and cholesterol by ethanol injection. Confocal microscopy showed better skin permeation and hair follicle accumulation of the niosomes compared to the PSO solution. The PSO-loaded niosomes inhibited 5α-reductase activity in DU-145 cells and hindered IL-6 activity in RAW 264.7 cells. These effects indicated the great potential of PSO-loaded niosomes to reduce hair loss. The hair scalp serum with PSO-loaded niosomes did not show irritation to reconstructed human skin. This formulation presented a significant decrease in the percentage of fallen hairs by 44.42% in the in vivo 60-second hair count experiment and a significant increase in the anagen to telogen (A/T) ratio (1.4-fold) in the TrichoScan evaluation after 8 weeks of treatment compared to the initial conditions, indicating the promising efficacy of PSO-loaded niosomes as a natural alternative for anti-hair loss therapy.
通过乙醇注入法，以吐温20和胆固醇为原料制备了负载南瓜籽油（PSO）的非离子表面活性剂囊泡。共聚焦显微镜显示，与PSO溶液相比，非离子表面活性剂囊泡具有更好的皮肤渗透性和毛囊蓄积性。负载PSO的非离子表面活性剂囊泡可抑制DU - 145细胞中的5α - 还原酶活性，并阻碍RAW 264.7细胞中的IL - 6活性。这些作用表明负载PSO的非离子表面活性剂囊泡在减少脱发方面具有巨大潜力。含有负载PSO的非离子表面活性剂囊泡的头皮血清对重建的人体皮肤未表现出刺激性。在体内60秒毛发计数实验中，该制剂使脱落毛发百分比显著降低了44.42%，并且在治疗8周后的TrichoScan评估中，与初始条件相比，生长期与休止期（A/T）比值显著增加（1.4倍），表明负载PSO的非离子表面活性剂囊泡作为抗脱发治疗的天然替代品具有良好的疗效。
