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乳杆菌属作为防腐剂和治疗剂在控制炎症性肠病中的抗炎和免疫调节作用。

Anti-inflammatory and immunomodulatory effects of Lactobacillus spp. as a preservative and therapeutic agent for IBD control.

机构信息

Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

出版信息

Immun Inflamm Dis. 2022 Jun;10(6):e635. doi: 10.1002/iid3.635.

DOI:10.1002/iid3.635
PMID:35634951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9119005/
Abstract

BACKGROUND

Probiotics have a beneficial effect on inflammatory responses and immune regulation, via Janus kinase/signal transduction and activator of transcription (JAK/STAT) and NF-κB signaling pathways. To evaluate the precise effects of Lactobacillus spp. as a protective and therapeutic agent, we aimed to investigate the efficacy of Lactobacillus spp. in modulating JAK/STAT and nuclear factor kappa B (NF-κB) inflammatory signaling pathways.

METHODS

A quantitative real-time polymerase chain reaction (qPCR) assay was used to analyze the expression of JAK/STAT and inflammatory genes (TIR-associated Protein [TIRAP], Interleukin 1 Receptor Associated Kinase[IRAK4], Nuclear factor-kappa B Essential Modulator [NEMO], and receptor interacting protein [RIP]) followed by treatment of the HT-29 cell line with sonicated pathogens before, after, and simultaneously with Lactobacillus spp. A cytokine assay was also used to evaluate interleukin (IL)-6 and IL-1β production after treatment with Lactobacillus spp.

RESULTS

Lactobacillus spp. downregulated JAK and TIRAP, IRAK4, NEMO, and RIP genes in the NF-κB pathway compared to sonicate-treated cells. The expression of STAT genes was different after treatment with probiotics. The production of IL-6 and IL-1β decreased after probiotic treatment.

CONCLUSIONS

Our Lactobacillus spp. cocktail showed anti-inflammatory effects on HT-29 cells by modulating JAK/STAT and NF-κB signaling pathways in all three treatment variants. Therefore, Lactobacillus spp. as a dietary supplement can both prevent and reduce inflammation-related diseases such as inflammatory bowel disease.

摘要

背景

益生菌通过 Janus 激酶/信号转导和转录激活因子（JAK/STAT）和 NF-κB 信号通路对炎症反应和免疫调节具有有益作用。为了评估乳杆菌属作为保护和治疗剂的确切效果，我们旨在研究乳杆菌属调节 JAK/STAT 和核因子 kappa B（NF-κB）炎症信号通路的功效。

方法

使用定量实时聚合酶链反应（qPCR）测定分析 JAK/STAT 和炎症基因（TIR 相关蛋白[TIRAP]、白细胞介素 1 受体相关激酶[IRAK4]、核因子-κB 必需调节剂[NEMO]和受体相互作用蛋白[RIP]）的表达，然后在乳杆菌属处理 HT-29 细胞系之前、之后和同时用超声处理的病原体处理。还使用细胞因子测定来评估处理乳杆菌属后白细胞介素（IL）-6 和 IL-1β 的产生。

结果

与超声处理的细胞相比，乳杆菌属下调了 NF-κB 通路中的 JAK 和 TIRAP、IRAK4、NEMO 和 RIP 基因。处理后 STAT 基因的表达不同。益生菌处理后 IL-6 和 IL-1β 的产生减少。

结论

我们的乳杆菌属混合物通过调节 JAK/STAT 和 NF-κB 信号通路在所有三种处理变体中显示出对 HT-29 细胞的抗炎作用。因此，作为膳食补充剂的乳杆菌属可以预防和减少炎症性肠病等炎症相关疾病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/c7d58019718d/IID3-10-e635-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/b7c02abab549/IID3-10-e635-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/939554906d87/IID3-10-e635-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/1bf6d34d7163/IID3-10-e635-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/c7d58019718d/IID3-10-e635-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/b7c02abab549/IID3-10-e635-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/939554906d87/IID3-10-e635-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/1bf6d34d7163/IID3-10-e635-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/9119005/c7d58019718d/IID3-10-e635-g002.jpg

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