Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
BMC Complement Med Ther. 2023 Sep 21;23(1):335. doi: 10.1186/s12906-023-04153-y.
IBD is considered an inflammatory disease with abnormal and exaggerated immune responses. To control the symptoms, different theraputic agents could be used, however, utilizing the agents with the least side effects could be important. Probiotics as beneficial microorganisms are one of the complementory theraputic agents that could be used to modulate inflammatory signaling pathways. In the current study, we aimed to identify the precise molecular effects of potential probiotics on signaling pathways involved in the development of inflammation.
A quantitative real-time polymerase chain reaction (qPCR) assay was used to analyze the expression of JAK /STAT (JAK1, JAK2, JAK3, TYK2, STAT1, STAT2, STAT3, STAT4, STAT5 and STAT6) and inflammatory genes (NEMO, TIRAP, IRAK, and RIP) after the HT -29 cell line treatment with the sonicated pathogens and potential probiotics. A cytokine assay was also used to evaluate IL -6 and IL -1β production after potential probiotic treatment.
The potential probiotic cocktail downregulated the JAK genes and TIRAP, IRAK4, NEMO, and RIP genes in the NF-kB pathway compared with cells that were treated with sonicated gram negative pathogens. The expression of STAT genes was different after potential probiotic treatment. The production of IL -6 and IL -1β decreased after potential probiotic treatment.
Considering the importance of controlling the symptoms of IBD to improve the life quality of the patients, using probiotic could be crucial. In the current study the studied native potential probiotic cocktails showed anti-inflammatory effects via modulation of JAK /STAT and NF-kB signaling pathways. This observation suggests that our native potential probiotics consumption could be useful in reducing intestinal inflammation.
IBD 被认为是一种炎症性疾病，具有异常和过度的免疫反应。为了控制症状，可以使用不同的治疗药物，然而，使用副作用最小的药物可能很重要。益生菌作为有益微生物是一种补充治疗药物，可以用来调节炎症信号通路。在本研究中，我们旨在确定潜在益生菌对参与炎症发展的信号通路的精确分子作用。
使用定量实时聚合酶链反应（qPCR）分析 HT-29 细胞系用超声病原体和潜在益生菌处理后 JAK/STAT（JAK1、JAK2、JAK3、TYK2、STAT1、STAT2、STAT3、STAT4、STAT5 和 STAT6）和炎症基因（NEMO、TIRAP、IRAK 和 RIP）的表达。还使用细胞因子分析评估潜在益生菌处理后 IL-6 和 IL-1β 的产生。
与用超声革兰氏阴性病原体处理的细胞相比，潜在益生菌混合物下调了 NF-kB 通路中的 JAK 基因和 TIRAP、IRAK4、NEMO 和 RIP 基因。STAT 基因的表达在潜在益生菌处理后不同。潜在益生菌处理后 IL-6 和 IL-1β 的产生减少。
鉴于控制 IBD 症状以提高患者生活质量的重要性，使用益生菌可能至关重要。在本研究中，研究的天然潜在益生菌混合物通过调节 JAK/STAT 和 NF-kB 信号通路显示出抗炎作用。这一观察结果表明，我们天然潜在益生菌的消费可能有助于减少肠道炎症。
