Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Semin Cell Dev Biol. 2022 Nov;131:117-123. doi: 10.1016/j.semcdb.2022.05.009. Epub 2022 May 27.
The formation of a central lumen in the human epiblast is a critical step for development. However, because the lumen forms in the epiblast coincident with implantation, the molecular and cellular events of this early lumenogenesis process cannot be studied in vivo. Recent developments using new model systems have revealed insight into the underpinnings of epiblast formation. To provide an up-to-date comprehensive review of human epiblast lumenogenesis, we highlight recent findings from human and mouse models with an emphasis on new molecular understanding of a newly described apicosome compartment, a novel 'formative' state of pluripotency that coordinates with epiblast polarization, and new evidence about the physical and polarized trafficking mechanisms contributing to lumenogenesis.
人胚外胚层中中央腔的形成是发育的关键步骤。然而，由于腔在胚外中形成与着床同时发生，因此无法在体内研究这个早期的腔发生过程的分子和细胞事件。最近使用新模型系统的发展揭示了对胚外形成基础的深入了解。为了提供对人类胚外层腔发生的最新全面综述，我们重点介绍了来自人类和小鼠模型的最新发现，强调了对新描述的顶体小泡隔室的新分子理解、协调与胚外极化的新型“形成性”多能性状态，以及有关有助于腔发生的物理和极化运输机制的新证据。
