Nurrohman Hamid, Carter Logan, Barnes Noah, Zehra Syeda, Singh Vineet, Tao Jinhui, Marshall Sally J, Marshall Grayson W
Missouri School of Dentistry and Oral Health, A.T. Still University, Kirksville, MO 63501, USA.
Preventive and Restorative Dental Sciences, University of California, San Francisco, CA 94143, USA.
Biomimetics (Basel). 2022 Apr 28;7(2):54. doi: 10.3390/biomimetics7020054.
The aim of this study was to investigate the effects of two process-directing agents (polyaspartic acid and osteopontin) used in a polymer-induced liquid-precursor (PILP) process on the remineralization of bacteria-induced enamel demineralization. Enamel demineralization lesions (depths of about 180-200 µm) were created and exposed to Streptococcus mutans, cultured with a 10% sucrose solution for 21 days, and remineralized using a PILP process (pH = 7.4, 14 days) with a calcium phosphate solution containing either polyaspartic acid or osteopontin in the presence or absence of fluoride (0.5 ppm). The specimens were examined under scanning electron microscopy. The fluoride was successfully incorporated into the PILP remineralization process for both polyaspartic acid and osteopontin. When the fluoride was added to the PILP remineralization solution, there was more uniform remineralization throughout the lesion than with either polyaspartic acid or osteopontin alone. However, in the absence of these process-directing agents, fluoride alone showed less remineralization with the formation of a predominantly surface-only layer. The PILP remineralization process relies on the ability of process-directing agents to stabilize calcium phosphate ions and holds promise for enamel lesion remineralization, and these agents, in the presence of fluoride, seem to play an important role as a booster or supplement in the continuation of remineralization by reducing the mineral gains at the surface layer.
本研究的目的是调查聚合物诱导液态前驱体（PILP）工艺中使用的两种工艺导向剂（聚天冬氨酸和骨桥蛋白）对细菌诱导的牙釉质脱矿再矿化的影响。制备牙釉质脱矿病变（深度约180 - 200 µm），使其暴露于变形链球菌，用10%蔗糖溶液培养21天，然后在有或没有氟化物（0.5 ppm）存在的情况下，使用含有聚天冬氨酸或骨桥蛋白的磷酸钙溶液通过PILP工艺（pH = 7.4，14天）进行再矿化。在扫描电子显微镜下检查标本。氟化物成功地融入了聚天冬氨酸和骨桥蛋白的PILP再矿化过程。当向PILP再矿化溶液中添加氟化物时，整个病变区域的再矿化比单独使用聚天冬氨酸或骨桥蛋白时更均匀。然而，在没有这些工艺导向剂的情况下，单独的氟化物显示出较少的再矿化，主要形成仅在表面的一层。PILP再矿化过程依赖于工艺导向剂稳定磷酸钙离子的能力，对牙釉质病变再矿化具有前景，并且这些试剂在氟化物存在的情况下，似乎通过减少表层的矿物质增加，在再矿化的持续过程中作为促进剂或补充剂发挥重要作用。
