Su Pin, Wicaksono Wisnu Adi, Li Chenggang, Michl Kristina, Berg Gabriele, Wang Dan, Xiao Youlun, Huang Renyan, Kang Houxiang, Zhang Deyong, Cernava Tomislav, Liu Yong
State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Graz University of Technology, Institute of Environmental Biotechnology, Graz, 8010, Austria.
Sci Data. 2022 Jun 1;9(1):254. doi: 10.1038/s41597-022-01320-7.
The plant microbiota plays crucial roles in sustaining plant health and productivity. Advancing plant microbiome research and designing sustainable practices for agriculture requires in-depth assessments of microorganisms associated with different host plants; however, there is little information on functional aspects of many microorganisms of interest. Therefore, we enriched microorganisms from the phyllosphere of 110 rice genotypes and subjected them to shotgun metagenomic sequencing to reconstruct bacterial genomes from the obtained datasets. The approach yielded a total of 1.34 terabases of shotgun-sequenced metagenomic data. By separately recovering bacterial genomes from each of the 110 rice genotypes, we recovered 569 non-redundant metagenome-assembled genomes (MAGs) with a completeness higher than 50% and contaminations less than 10%. The MAGs were primarily assigned to Alphaproteobacteria, Gammaproteobacteria, and Bacteroidia. The presented data provides an extended basis for microbiome analyses of plant-associated microorganisms. It is complemented by detailed metadata to facilitate implementations in ecological studies, biotechnological mining approaches, and comparative assessments with genomes or MAGs from other studies.
植物微生物群落在维持植物健康和生产力方面发挥着关键作用。推进植物微生物组研究并设计可持续的农业实践需要深入评估与不同寄主植物相关的微生物；然而，关于许多感兴趣的微生物的功能方面的信息却很少。因此，我们从110个水稻基因型的叶际中富集微生物，并对其进行鸟枪法宏基因组测序，以便从获得的数据集中重建细菌基因组。该方法共产生了1.34太字节的鸟枪法测序宏基因组数据。通过分别从110个水稻基因型中回收细菌基因组，我们获得了569个非冗余的宏基因组组装基因组（MAG），其完整性高于50%，污染率低于10%。这些MAG主要属于α-变形菌纲、γ-变形菌纲和拟杆菌纲。所呈现的数据为植物相关微生物的微生物组分析提供了扩展基础。它还辅以详细的元数据，以促进在生态研究、生物技术挖掘方法以及与其他研究的基因组或MAG进行比较评估中的应用。
