Hsieh Hui-Shan, Gong Yu-Nong, Chi Chih-Yu, Hsieh Sen-Yung, Chen Wei-Ting, Ku Cheng-Lung, Cheng Hao-Tsai, Lin Lyndsey, Sung Chang Mu
Department of Otolaryngology-Head and Neck Surgery, Xiamen Chang Gung Hospital, Xiamen, China.
Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Sci Rep. 2022 Jun 1;12(1):9126. doi: 10.1038/s41598-022-13289-9.
Autoantibodies against interferon-gamma (AutoAbs-IFN-γ) can cause the immunodeficiency condition following various opportunistic infections. Gut microbiota can affect the human immune system in many ways. Many studies have shown that gut dysbiosis was associated with some immune diseases, such as autoimmune diseases and human immunodeficiency virus (HIV) infection, while its relationship at anti-IFN-γ AAbs remains unknown. We aimed to identify the anti-IFN-γ AAbs specific microbiome and the possible association with immunodeficiency. We profiled fecal microbiome for two cohorts of forty subjects, including seven patients with anti-IFN-γ AAbs and 33 individuals with competent immune. The study shows that patients with anti-IFN-γ AAbs have characterized the gut microbiome and have lower alpha diversity indexes than healthy controls (HC). There are significant differences in the microbiome structure at both the family and genera level between the two cohorts. The anti-IFN-γ AAbs cohort featured some microbiome such as Clostridium, including the possible opportunistic pathogen and fewer genera including Bacteroides, Ruminococcus, and Faecalibacterium, some of them with possible immune-related genera. The PICRUSt2 pathway demonstrated the decreased abundance of some immune-related pathways and one potential pathway related to the immune alternations in the anti- IFN-γ AAbs cohort. This was the first study to examine the gut microbiome characteristics in patients with anti-IFN-γ AAbs. It could be involved in the pathogenesis of anti-IFN-γ AAbs and contribute to the derived immune condition in this disease. This could lead to new strategies for treating and preventing patients suffering from this disease.
抗干扰素-γ自身抗体（AutoAbs-IFN-γ）可导致各种机会性感染后的免疫缺陷状态。肠道微生物群可通过多种方式影响人体免疫系统。许多研究表明，肠道微生物失调与一些免疫疾病有关，如自身免疫性疾病和人类免疫缺陷病毒（HIV）感染，但其与抗IFN-γ自身抗体的关系尚不清楚。我们旨在确定抗IFN-γ自身抗体特异性微生物群及其与免疫缺陷的可能关联。我们对两个队列的40名受试者的粪便微生物群进行了分析，其中包括7名抗IFN-γ自身抗体患者和33名免疫功能正常的个体。研究表明，抗IFN-γ自身抗体患者具有特定的肠道微生物群特征，其α多样性指数低于健康对照（HC）。两个队列在科和属水平上的微生物群结构存在显著差异。抗IFN-γ自身抗体队列具有一些微生物群，如梭菌属，包括可能的机会性病原体，以及较少的属，如拟杆菌属、瘤胃球菌属和粪杆菌属，其中一些属可能与免疫相关。PICRUSt2通路显示抗IFN-γ自身抗体队列中一些免疫相关通路的丰度降低，以及一条与免疫改变相关的潜在通路。这是第一项研究抗IFN-γ自身抗体患者肠道微生物群特征的研究。它可能参与抗IFN-γ自身抗体的发病机制，并导致该疾病的免疫状态。这可能为治疗和预防患有这种疾病的患者带来新的策略。
