State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
Colloids Surf B Biointerfaces. 2022 Aug;216:112596. doi: 10.1016/j.colsurfb.2022.112596. Epub 2022 May 25.
Synergistic functionalization of interface coagulation stimulation and liquid absorption capacity is the key to improve the hemostatic efficiency of hemostats. Herein, we prepared a graphene-ophicalcite (OPH) heterogeneous composite sponge (GOCS) by using the heterogeneous gradient composite strategy. The sponge took cross-linked graphene sponge (CGS) as the main skeleton, allowing the OPH to be controllably positioned on the surface of GOCS. The heterogeneous strategy gave full play to the advantages of the material. On the one hand, GOCS had excellent liquid absorption ability, which enriched blood cells and other coagulation components at the wound interface after contacting blood. On the other hand, the OPH at the interface obviously activated platelets and rapidly triggered coagulation cascade reactions, exhibiting fast response and feedback characteristics for coagulation signals. Under the synergistic effects, the blood clotting index value of GOCS was reduced to 33.87 ± 9.97%, which was significantly lower than those of OPH (46.33 ± 16.85%) and CGS (67.53 ± 5.35%). Importantly, GOCS rapidly stopped bleeding within 51 s in the rat femoral artery model, suggesting its great potential in the field of hemostasis. Therefore, this study provides a new idea for the design and preparation of hemostatic materials via heterogeneous strategy.
界面凝固刺激和液体吸收能力的协同功能化是提高止血剂止血效率的关键。在此，我们通过非均匀梯度复合策略制备了一种石墨烯-沸石（OPH）异质复合海绵（GOCS）。该海绵以交联石墨烯海绵（CGS）为主要骨架，使 OPH 可在 GOCS 表面进行可控定位。该非均匀策略充分发挥了材料的优势。一方面，GOCS 具有优异的液体吸收能力，在接触血液后，可在伤口界面处富集血细胞和其他凝血成分。另一方面，界面处的 OPH 明显激活血小板并迅速引发凝血级联反应，表现出对凝血信号的快速响应和反馈特性。在协同作用下，GOCS 的凝血指数值降低至 33.87±9.97%，明显低于 OPH（46.33±16.85%）和 CGS（67.53±5.35%）。重要的是，GOCS 在大鼠股动脉模型中可在 51s 内迅速止血，表明其在止血领域具有巨大的潜力。因此，本研究为通过非均匀策略设计和制备止血材料提供了新的思路。
