Yan Ying, Tang Pan, Zhang Xiong, Wang Dan, Peng Mingyou, Yan Xiaoyan, Hu Zuquan, Tang Lei, Hao Xiaojiang
State Key Laboratory of Functions and Applications of Medicinal Plants & College of Pharmacy, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R&D, Guizhou Medical University, Guiyang 550014, China; School of Medicine and Health Management, Guizhou Medical University, Guiyang 550025, China.
State Key Laboratory of Functions and Applications of Medicinal Plants & College of Pharmacy, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R&D, Guizhou Medical University, Guiyang 550014, China.
Fitoterapia. 2022 Sep;161:105225. doi: 10.1016/j.fitote.2022.105225. Epub 2022 May 31.
Tobacco mosaic virus (TMV) is known to infect a wide range of plants, resulting in reduced yield and productivity. Novel, effective, and plant-based pesticides are required to protect plants against TMV infection. To identify novel anti-TMV agents from natural sources, we systematically studied the roots of Cynanchum paniculatum and isolated six new seco-pregnane C steroidal glycosides, along with 14 known compounds. Their structures were elucidated by comprehensive spectroscopic data analysis. The anti-TMV activity of compounds were screened using the half-leaf method. Biological tests revealed that compounds 1, 2, 5, 9, 10, 15, and 16 displayed significant anti-TMV activities compared with the positive control ningnanmycin. In addition, reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis confirmed the antiviral activity of these compounds, as evident from reduced TMV coat protein (TMV-CP) gene replication and TMV-CP protein expression. These compounds downregulated the expression of NtHsp70-1 and NtHsp70-261, indicating that these steroidal glycosides possibly inhibit the TMV infection by suppressing the expression of NtHsp70-1 and NtHsp70-061 expression.
烟草花叶病毒（TMV）已知会感染多种植物，导致产量和生产力下降。需要新型、有效且基于植物的农药来保护植物免受TMV感染。为了从天然来源中鉴定新型抗TMV药剂，我们系统地研究了徐长卿的根，并分离出6种新的裂孕甾烷C甾体苷以及14种已知化合物。通过综合光谱数据分析阐明了它们的结构。使用半叶法筛选化合物的抗TMV活性。生物学测试表明，与阳性对照宁南霉素相比，化合物1、2、5、9、10、15和16表现出显著的抗TMV活性。此外，逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析证实了这些化合物的抗病毒活性，这从TMV外壳蛋白（TMV-CP）基因复制减少和TMV-CP蛋白表达中可以明显看出。这些化合物下调了NtHsp70-1和NtHsp70-261的表达，表明这些甾体苷可能通过抑制NtHsp70-1和NtHsp70-061的表达来抑制TMV感染。
