白薇根中的裂孕甾烷甾体糖苷及其抗烟草花叶病毒活性。
Seco-pregnane steroidal glycosides from the roots of Cynanchum atratum and their anti-TMV activity.
作者信息
Yan Ying, Zhang Jian-xin, Liu Kai-xing, Huang Tao, Yan Chen, Huang Lie-jun, Liu Sheng, Mu Shu-zhen, Hao Xiao-jiang
机构信息
Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, People's Republic of China; The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
出版信息
Fitoterapia. 2014 Sep;97:50-63. doi: 10.1016/j.fitote.2014.03.027. Epub 2014 Apr 5.
Fifteen new seco-pregnane steroidal glycosides cynanosides A-O (1-15) together with twenty-seven known ones were isolated from the roots of Cynanchum atratum. The structures of 1-15 were determined by extensive analysis of spectroscopic data. The anti-tobacco mosaic virus (TMV) activity of these steroidal glycosides was screened by the conventional half-leaf method, enzyme-linked immunosorbent assay, and Western blot methods, most of them showed potent anti-TMV activity. Among them, compounds 1, 7, 13, 28 and 31 showed significantly anti-TMV activity with an IC50 value of 20.5, 18.6, 22.0, 19.2 and 22.2 μg/mL, respectively, and were much more effective than the positive control, ningnanmycin (IC50=49.6 μg/mL).
从白薇根中分离得到15个新的裂孕甾烷甾体糖苷类化合物苦玄参苷A - O（1 - 15）以及27个已知化合物。通过对光谱数据的广泛分析确定了1 - 15的结构。采用常规半叶法、酶联免疫吸附测定法和蛋白质印迹法对这些甾体糖苷的抗烟草花叶病毒（TMV）活性进行筛选，其中大多数表现出较强的抗TMV活性。其中，化合物1、7、13、28和31表现出显著的抗TMV活性，IC50值分别为20.5、18.6、22.0、19.2和22.2 μg/mL，且比阳性对照宁南霉素（IC50 = 49.6 μg/mL）更有效。
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