从.中分离得到 12 个新的螺甾烷型糖苷。
Twelve New Seco-Pregnane Glycosides from .
机构信息
School of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
Molecules. 2022 Aug 26;27(17):5500. doi: 10.3390/molecules27175500.
For our interest in the potential biologically active and structurally unique steroidal glycosides, continued phytochemical investigation of was carried out; twelve new seco-pregnane glycosides, cynataihosides I-L (-), M-T (-), and two known glycosides, glaucoside A () and atratcynoside F (), were isolated from the 95% ethanol extract of . Two new aglycones were found among compounds , , , and . The structures of the glycosides were elucidated based on 1D and 2D NMR spectroscopic data, HR-ESI-MS analysis, and chemical evidence. The cytotoxicity of compounds against three human tumor cell lines (HL60, THP-1, and PC-3) were evaluated by MTT assay. Compound displayed significant cytotoxicity against THP-1 and PC-3 cell line with IC values of 5.08 and 22.75 μm, respectively. Compounds and exhibited moderate and selective cytotoxicity on HL60 and THP-1 with IC values of 17.78 and 16.02 μm, respectively.
为了研究具有潜在生物活性和独特结构的甾体糖苷，我们对 进行了进一步的植物化学研究；从 95%乙醇提取物中分离得到了 12 种新的孕烷糖苷，分别为 cynataihosides I-L(-)、M-T(-)，以及两种已知糖苷，glaucoside A()和 atratcynoside F()。在化合物 、 、 、 中发现了两种新的苷元。根据 1D 和 2D NMR 波谱数据、高分辨电喷雾质谱分析和化学证据确定了糖苷的结构。采用 MTT 法评价了化合物对三种人肿瘤细胞系（HL60、THP-1 和 PC-3）的细胞毒性。化合物 对 THP-1 和 PC-3 细胞系表现出显著的细胞毒性，IC 值分别为 5.08 和 22.75 μm。化合物 、 对 HL60 和 THP-1 具有中等选择性细胞毒性，IC 值分别为 17.78 和 16.02 μm。
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