Takuissu Guy Roussel, Fonkoua Martin, Mandob Damaris, Ngoumen Dany, Ambamba Dupon, Nanhah Jules, Ngondi Judith Laure
Centre for Food, Food Security and Nutrition Research (CRASAN), Institute for the Medical Research and Medicinal Plant Studies (IMPM), Ministry of Scientific Research and Innovation, Cameroon.
Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon.
Metabol Open. 2022 May 25;14:100192. doi: 10.1016/j.metop.2022.100192. eCollection 2022 Jun.
The management of hyperglycemia and oxidative stress is a key point in the control of diabetes and its complications. The work evaluated the hypoglycemiant and antioxidant properties of pulp extracts of fruits. The acute toxicity was conducted for 14 days at the dose of 2000 mg/kg via oral gavage. Diabetes was induced in rats by intravenous administration of streptozotocin (50 mg/kg). The effect of the extract on blood glucose levels of diabetic rats was monitored at 2h after administration; and on the 7th and 14th days of treatment (single dose of 400 mg/kg/day). After 2 weeks of treatment, the rats were sacrificed. The oxidative stress markers (Hydroperoxides, Malonedialdehydes, Superoxide Dismutase, Catalase, and Total Antioxidant Capacity) and hepato/nephrotoxicity markers (Alanine Amino Transferase, urea, and creatinine) were determined. The hydroethanolic extract of fruits has a lethal dose upper than 2000 mg/kg. It significantly decreased glucose levels by 28.29% and 84.86% respectively after 2h and 14th days of treatment. The extract increased the antioxidant status and decrease oxidative stress, especially in the pancreas. This study suggests that the hydroethanolic pulp extract of fruits is a good hypoglycemiant and antioxidant agent and could be a potential source of compounds for diabetes management.
高血糖和氧化应激的管理是控制糖尿病及其并发症的关键所在。这项研究评估了水果果肉提取物的降血糖和抗氧化特性。通过灌胃以2000毫克/千克的剂量进行了为期14天的急性毒性试验。通过静脉注射链脲佐菌素（50毫克/千克）诱导大鼠患糖尿病。给药后2小时监测提取物对糖尿病大鼠血糖水平的影响；并在治疗的第7天和第14天（单剂量400毫克/千克/天）进行监测。治疗2周后，处死大鼠。测定氧化应激标志物（氢过氧化物、丙二醛、超氧化物歧化酶、过氧化氢酶和总抗氧化能力）以及肝/肾毒性标志物（丙氨酸氨基转移酶、尿素和肌酐）。水果的水乙醇提取物的致死剂量高于2000毫克/千克。治疗2小时和14天后，它分别显著降低血糖水平28.29%和84.86%。该提取物提高了抗氧化状态并降低了氧化应激，尤其是在胰腺中。这项研究表明，水果的水乙醇果肉提取物是一种良好的降血糖和抗氧化剂，可能是用于糖尿病管理的化合物的潜在来源。
